Traditional diagnostic tests for chronic diseases are expensive and require a specialized laboratory, therefore limiting their use for point-of-care (PoC) testing. To address this gap, we developed a method for rapid and low-cost C-reactive protein (CRP) detection from blood by integrating a paper-based microfluidic immunoassay with a smartphone (CRP-Chip). We chose CRP for this initial development because it is a strong biomarker of prognosis in chronic heart and kidney disease. The microfluidic immunoassay is realized by lateral flow and gold nanoparticle-based colorimetric detection of the target protein. The test image signal is acquired and analyzed using a commercial smartphone with an attached microlens and a 3D-printed chip–phone interface. The CRP-Chip was validated for detecting CRP in blood samples from chronic kidney disease patients and healthy subjects. The linear detection range of the CRP-Chip is up to 2 μg/mL and the detection limit is 54 ng/mL. The CRP-Chip test result yields high reproducibility and is consistent with the standard ELISA kit. A single CRP-Chip can perform the test in triplicate on a single chip within 15 min for less than 50 US cents of material cost. This CRP-Chip with attractive features of low-cost, fast test speed, and integrated easy operation with smartphones has the potential to enable future clinical PoC chronic disease diagnosis and risk stratification by parallel measurements of a panel of protein biomarkers.

The waste of coal resources, a complicated production process and slow mining speed seriously restrict the rapid development of longwall mining. To achieve effective mining, an innovative noncoal pillar mining approach (i.e., Gob-side Entry Retaining by Roof Cutting (GERRC)) was introduced. The mechanism of the GERRC approach and its three key technologies (i.e., roof support technology, directional presplit cumulative blasting technology and surrounding rock control technology) were studied by theoretical analysis, numerical simulation, laboratory and field experiments. The new approach was finally tested under medium-thick coal seam and compound roof conditions. The results show that the directional presplit cumulative blasting technology can effectively control the damage evolution in the roof rock, maintain the integrity of the entry roof and contribute the gob roof to the cave in time. The support technologies in different roof movement stages can control the entry surroundings, and the final section of the retained entry met the safety production requirements. The test results suggested that the proposed approach for coal effective mining is feasible, and the introduced key technologies and design methods potentially produce reasonable values for applications of pillarless mining in similar projects.

Gob-side entry retaining by roof cutting (GERRC) employed in a deep inclined thick coal seam (DITCS) can not only increase economic benefits and coal recovery, but also optimize surrounding rock structure. In accordance with the principles of GERRC, the technology of GERRC in DITCS is introduced and a roof-cutting mechanical model of GERRC is proposed to determine the key parameters of the depth and angle of RC. The results show that the greater the RC angle, the easier the caving of the goaf roof, but the length of cantilever beam increases. The depth of RC should account for the dip angle of the coal seam when the angle is above 20°. Increasing the coal seam dip angle could reduce the volume of rock falling of the goaf roof, but increase the filling height of the upper gangue to slide down. According to numerical model analysis of the stress and displacement of surrounding rock at different depths and angles of RC, when the depth of RC increased from 9 m to 13 m, the distance between the stress concentration zone and the coal side is increased. When the angle of RC increased from 0° to 20°, the value of roof separation is decreased. GERRC was applied in a DITCS with 11 m depth and 20° RC angle, and the field-measured data verified the conclusions of the numerical model.

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Directional presplit blasting (DPB) technology for an innovative no-pillar mining approach is introduced. First, a mechanical model is established to analyze crack initiation and coalescence using the new technology. The explosion pressure formula containing tress concentration factor explains the relationship between the quantity of explosives and the crack initiation and coalescence. Subsequently, a dynamic finite element simulation method is used to study the stress and damage evolution process of the blasthole wall in ordinary explosion and DPB. The simulation results indicate that the stress concentration factor is approximately 4c10 for the same quantity of explosives. Finally, a field test was conducted in the Fucheng coal mine of China. Based on the field test, the concept of cracking ability is proposed, the relationship between the quantity of explosives and cracking ability is determined, and the quantity of explosives is divided into four stages that are compatible with the roof rock strength. The results can provide some clinical guidance for application of DPB in other projects of the gob-side entry retaining by roof cutting.