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The aim of this study was to estimate the acute effects of low dose 12C6+ ions or X-ray radiation on human immune function. The human peripheral blood lymphocytes (HPBL) of seven healthy donors were exposed to 0.05Gy 12C6+ ions or X-ray radiation and cell responses were measured at 24 hours after exposure. The cytotoxic activities of HPBL were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT); the percentages of T and NK cells subsets were detected by flow cytometry; mRNA expression of interleukin (IL)-2, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were examined by real time quantitative RT-PCR (qRT-PCR); and these cytokines protein levels in supernatant of cultured cells were assayed by enzyme-linked immunosorbent assays (ELISA). The results showed that the cytotoxic activity of HPBL, mRNA expression of IL-2, IFN-γ and TNF-α in HPBL and their protein levels in supernatant were significantly increased at 24 hours after exposure to 0.05Gy 12C6+ ions radiation and the effects were stronger than observed for X-ray exposure. However, there was no significant change in the percentage of T and NK cells subsets of HPBL. These results suggested that 0.05Gy high linear energy transfer (LET) 12C6+ radiation was a more effective approach to host immune enhancement than that of low LET X-ray. We conclude that cytokines production might be used as sensitive indicators of acute response to LDI.

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Chinese government puts forward a concept: five in one, putting environment in the same significant position as economy, politics, society and culture, which highlights the significance of environment. With the continuous increase of environmental awareness, many scholars pay more attention to green finance. However, the researches based on economic or social aspects, mostly stay on the theoretical level without studying how much people know about this concept. This paper, however, based on the questionnaire on Guangdong Undergraduates, aims to know about the deviation between undergraduates' awareness of the importance of green finance and the real situation. The results turn out that there are significant deviations in the following areas: concept, methods, reasons, purpose and representative principles, etc. Based on the results, the author tries to explain the reason of the existence of these deviations and the connection of enhancing education of environmental protection.