The problem of salinity stress in agricultural land is increasing rapidly, mainly due to climate change. Salinity in the soil has a detrimental effect on the root growth of rice and reduces the ability of the plant to absorb water and other nutrients from the soil resulting in stunted growth. The application of silicate fertilizers is an effort to reduce the negative effects of salinity stress. Absorption of beneficial element silicon (Si) by rice plants can reduce salinity stress. The objective of this research is to analyze the effect of calcium silicate on rice root growth and its tolerance to salinity stress. This research was conducted with the addition of silicate fertilizer consisting of three levels of CaSiO3 (0 mM, 2 mM, 4 mM) in red rice (Oryza sativa L. ‘Sembada Merah’) under salinity stress treatment at the level of 0 dS m-1(control), 3 dS m-1 (low), 7 dS m-1 (moderate) and 10 dS m-1 (high). The treatment with a salinity level of 10 dS m-1 reduced root length, fresh and dry weight of the root. The addition of calcium silicate in salinity stress conditions was able to improve the root anatomical characteristics of rice ‘Sembada Merah’ by increasing the epidermis thickness, cortex thickness, stele diameter and root diameter. Silicate fertilizer is indicated to play a role in increasing suberin and lignin to form apoplast defenses in order to prevent the entry of Na+ ions into the stele.
Viability and vigor are components of seed quality assessment which are influenced by internal and external factors of the seed, i.e., salinity and temperature. Salinity is related to the interaction between osmotic pressure and seed germination. This study used 3 types of seeds, they are peanuts (Arachis hypogaea L.), mung bean (Vigna radiata (L.) R. Wilczek), and corn (Zea mays L.). The purposes of this study were to determine seed viability by using Triphenyl tetrazolium chloride (TTC), to analyze the effects of different osmotic pressures and temperatures on seed viability and vigor. Peanut seed viability test was conducted by using 1% TTC solution. Testing the effect of osmotic pressure on seed viability and vigor was conducted by using various concentrations of NaCl (0.01 M; 0.05 M; 0.5 M; 0.1 M; 1 M). The germination test of the three seeds was conducted by using temperatures of 30°C and 35°C, each with three replications. The test parameters measured included germination, germination rate, germination speed index, vigor index, simultaneous growth, and the speed of seed growth. The results showed that TTC solution was effectively used to determine seed viability. Mung bean had the highest germination rate, LP, IKP, IV, simultaneous growth, and speed of seed growth compared to the other tested seeds in all salinity and temperature treatments. The 30 ⁰C temperature treatment resulted in the best viability and seed vigor for the three tested plants. Treatments of temperature and salinity affect the quality of the three plant seeds which can then be used to determine the quality of peanut, mung bean, and corn seeds.
Keywords: germination, salinity, triphenyl tetrazolium chloride, viability, vigour
Climate change causes an increase in soil salinity, hence reduce rice growth and productivity. The application of silicate fertilizer as a source of silicon (Si) can be encouraged to enhance the rice tolerance against biotic and abiotic stresses. This study aimed to analyze the potential of silicate fertilizer to alleviate the salinity stress on red rice. A pot experiments was conducted to observe the physiological and biochemical aspects. Rice seedlings were treated with three doses of rice husk ash/RHA (0, 4, and 8 tons ha-1), and three levels of salinity stress, namely control (0 dS m-1), low (3 dS m-1), moderate (7 dS m-1), and high (10 dS m-1). The observed parameters were plant height, number of tillers, plant biomass, activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT), levels of chlorophyll, carotenoid and proline. The results showed the growth of red rice ‘Sembada Merah’ was inhibited by moderate and high salinity stress as indicated by a decrease in plant height, number of tillers and plant biomass. Photosynthetic pigment chlorophyll and carotenoid levels decreased with the increasing salinity stress. RHA application increased SOD activity as a response to enhance salinity stress tolerance. At doses of 4 t ha-1, RHA could maintain rice growth up to moderate salinity stress.
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