Investigating the deformation of tissue architecture is one of the most important clinical methods for cancer diagnosis. Optical methods are now widely developed for rapid, precise, and real-time assessment of these alterations at the microscopic scale. One of the proposed methods is enhanced backscattering (EBS) technique that allows in-vivo measurement of the optical scattering characteristics. Here, EBS technique is employed to evaluate the optical anisotropy of human epithelial tissues as a measure to distinguish between normal and cancerous one. Orientation dependence of the mean scattering length is assessed in healthy and cancerous tissues of five different human organs i. e. uterus, bladder, colon, kidney, and liver. Helicity preserving channel and rotating ground glass diffuser are utilized to eliminate the polarization induced anisotropy and the background speckle noises respectively. Analysis of the backscattering cones recorded by a high-resolution CCD camera reveals the modification of the strength and degree of optical anisotropy in different tissues during cancer progression. Pathology data affirm the correlation between the experimental results and the morphological alteration of the epithelial cells in each carcinoma type. In general, tissues with fibrous constructional cells are subject to a decrease in anisotropy due to cancer, whereas those with cuboidal cells experience an increase in anisotropy. This complementary information enhances the potency of the EBS technique as a fast, non-destructive, and easily accessible tool for real-time tissue diagnosis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.