Zinc distribution and speciation within different organs (root, petiole, and leaf) of the edible plant Eruca vesicaria L. Cavalieri were determined using synchrotron microbeam X-ray techniques (XRF microtomography and mu-XANES) for plants grown in polluted soil with or without compost amendment. Data on soil derived from different extraction procedures and using mu-XANES analyses on rhizospheric soil indicated that compost amendment did not significantly influence the Zn speciation and availability in soil. However, major differences were observed within the plants. Plants grown in the presence of compost were able to partly block zinc immediately outside the root endodermis in the form of zinc-phytate, while a smaller Zn fraction was allowed to xylem transport as zinc-citrate. In the leaves, zinc was largely excluded from leaf cells, and about approximately 50% was in the form of phosphate precipitates, and the other 50% was complexed by cysteine and histidine residues. The reported data provide new information concerning the mechanisms of zinc tolerance in E. vesicaria L. Cavalieri, a very common edible plant in Mediterranean regions, and on the role of compost in influencing the molecular strategies involved in zinc uptake and detoxification.
The potential use of biochar from olive mill waste for in situ remediation of metal contaminated soils was evaluated. Biochar was mixed with metal contaminated soil originating from the vicinity of an old zinc smelter. Soil-biochar mixtures were equilibrated for 30 and 90 days. At these time points, Ca(NO3)2 exchangeable metals were determined, and effects of the biochar amendment on soil toxicity were investigated using plants, bacteria, and earthworms. Bean (Phaseolus vulgaris) growth, metal content, antioxidative enzymes activities, and soluble protein contents were determined. Furthermore, effects on soil microbial communities (activity, diversity, richness) were examined using Biolog ECOplates. After 120 days of soil-biochar equilibration, effects on weight and reproduction of Eisenia foetida were evaluated. With increasing biochar application rate and equilibration period, Ca(NO3)2 exchangeable metals decreased, and growth of bean plants improved; leaf metal contents reduced, the activities of antioxidative stress enzymes decreased, and soluble protein contents increased. Soil microbial activity, richness, and diversity were augmented. Earthworm mortality lowered, and their growth and reproduction showed increasing trends.
Suitable plant species are able to accumulate heavy metals and to produce biomass useful for non-food purposes. In this study, three endemic Mediterranean plant species, Atriplex halimus, Portulaca oleracea and Medicago lupulina were grown hydroponically to assess their potential use in phytoremediation and biomass production. The experiment was carried out in a growth chamber using half strength Hoagland's solutions separately spiked with 5 concentrations of Pb and Zn (5, 10, 25, 50, and 100 mg L(-1)), and 3 concentrations of Ni (1, 2 and 5 mg L(-1)). Shoot and root biomass were determined and analyzed for their metals contents. A. halimus and M. lupulina gave high shoot biomass with relatively low metal translocation to the above ground parts. Metals uptake was a function of both metals and plant species. It is worth noting that M. lupulina was the only tested plant able to grow in treatment Pb50 and to accumulate significant amount of metal in roots. Plant metal uptake efficiency ranked as follows: A. halimus > M. lupulina > P. oleracea. Due to its high biomass production and the relatively high roots metal contents, A. halimus and M. lupulina could be successfully used in phytoremediation, and in phytostabilization, in particular.
Metals are very common contaminants in the soil. High-yielding biomass crops offer good potential for the phytoremediation of soils contaminated with heavy metals. Biomass fuel crops grown on contaminated land have several advantages as site remediation combined with bioenergy production. In this context, two energy crops, Sorghum bicolor and Carthamus tinctorius, were grown hydroponically to assess their potential use in phytoremediation of nickel (Ni), lead (Pb) and zinc (Zn) and biomass production. The experiment was carried out in a growth chamber using half-strength Hoagland's solution spiked separately with five concentrations for Ni, Pb and Zn (between 5 and 100 mg L -1 ). Shoot and root biomass were determined and analyzed for their metals contents. Results showed that the tested plants were able to uptake Ni, Pb and Zn. Furthermore, roots accumulated more metals than shoots. Ni seems to be more toxic than Zn and Pb. In fact, both species were unable to grow at Ni concentration above 10 mg L -1 . Metal toxicity ranked as follows: Ni [ Zn [ Pb. High toxicity symptoms and biomass reduction were observed at concentrations of Pb and Zn above 25 mg L -1 for both species. S. bicolor was more efficient than C. tinctorius in metal uptake due to the high biomass production and the relatively high shoot concentration of metal. S. bicolor could be successfully used in phytoremediation applications in marginal soils with moderately heavy metal contamination. However, results obtained through the hydroponic experiment need to be confirmed by field experiments.
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