Dose-dependent effects of selenium on growth and physiological trait of wheat seedlings (Triticum aestivum L. cv Han NO.7086) exposed to cold stress are reported. Responses of seedlings were different depending on the Se concentration. The treatments with 0.5 and 1.0 mg Se kg(-1) significantly increased biomass and chlorophyll content of seedlings. However, the treatments at 2.0 and 3.0 mg Se kg(-1) only induced an evident increase in chlorophyll content and did not promote biomass accumulation of seedlings. Antioxidant compounds content (anthocyanins, flavonoids, and phenolic compounds) and antioxidant enzymes' activities (peroxidase and catalase) increased by different Se treatments, while only the treatment with 1.0 mg Se kg(-1) induced a significant reduce in malondialdehyde content and the rate of superoxide radical production of wheat seedlings. The results of this study demonstrated that Se supply could increase antioxidant capacity of seedlings, and optimal Se supply reduced production of free radicals, membrane lipid peroxidation, and promoted biomass accumulation.
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