Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiologic agent of porcine reproductive and respiratory syndrome (PRRS), which can evolve continuously by random mutation or intragenic recombination. Here we report the complete genomic sequence of a PRRSV variant with nucleotide acid deletions and insertions in the nonstructural protein 2 (nsp2) gene and a possible recombination event between a modified live virus (MLV) vaccine strain and a prototype Chinese field strain. P orcine reproductive and respiratory syndrome (PRRS) outbreaks occur worldwide and cause significant economic losses in the swine industry (6, 7). Even though modified live virus (MLV) vaccines were shown to provide solid protection against PRRSV infection, there were substantial barriers to the use of MLV vaccines in the field (2, 5). Considering the extensive use of MLV vaccines for preventing and controlling PRRS in mainland China, the appearance of a natural recombination event between a vaccine strain and a field strain is noteworthy.Serum and tissue samples were collected from suspected growing pigs in Guangdong Province in 2011. Infected pigs were about 10 weeks old and displayed symptoms similar to those of original PRRS, including severe respiratory problems, diarrhea, and poor growth. In addition, this disease resulted in a ϳ2.2% mortality rate on this farm. A porcine reproductive and respiratory syndrome virus (PRRSV) field isolate, named GM2, was propagated on Marc-145 cells, and total viral RNA was extracted from the serum and infected cell culture and then used separately for the amplification of the genome. Basically, 16 primer pairs were used to generate overlapping amplicons by reverse transcription-PCR (RT-PCR) as reported previously (8), and 3=-terminal sequences of GM2 were obtained by a 3= rapid amplification of cDNA ends (RACE kit; TaKaRa). The PCR products were cloned into pMD19-T vector (TaKaRa), sequenced three times using an ABI 3730 Sanger-based genetic analyzer, and assembled using DNAStar version 7.0 to obtain the complete genome sequence. The completed sequence showed that, excluding the poly(A) tail, the genomic sequence of GM2 was 15,513 nucleotides in length. Genetic analysis demonstrated that GM2 shared 90.2% genome similarity with VR-2332, but a lower degree of genetic homology (87.7%) was observed than with the representative highly pathogenic PRRSV JXA1 strain. Compared with the VR-2332 strain, 36-amino-acid insertions and two continuous amino acid deletions in the nonstructural protein 2 (nsp2) region of GM2 were found. However, GM2 shared a very low identity with the SP strain (68.8%) and the Japanese strain EDRD-1 (70.5%), which was characterized by similar insertions within nsp2.Recombination events were detected using both Simplot version 3.5.1 (3) and the recombination detection program version 4.1.3 (RDP4) (4). The results indicated that GM2 was a potential recombinant between the MLV RespPRRS/Repro vaccine strain and a recently emerging prototype Chinese field strain, named QYYZ. P...

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration with high mortality rates in swine. It has become increasingly problematic in China. Since the nucleocapsid (N) protein is highly conserved, it is a candidate protein for early diagnosis and vaccine development. In this study, the N genes of 15 PEDV strains were amplified by RT-PCR and cloned into the pMT-19T vector, sequenced, and compared to each other as well as to PEDV reference strains. The nucleotide sequences of the N gene of the Chinese PEDV strains consist of 1326 nucleotides and encode a 441-aa-long peptide. The nucleotide sequences of the fifteen PEDV strains in our study were 96.1-100 % identical to each other, and the deduced amino acid sequences were 94.8-100 % identical. Sequence comparison with other PEDV strains selected from GenBank revealed that their nucleotide sequences were 94.2-99.7 % identical to those of the Chinese PEDV strains, and their deduced amino acid sequences were 94.1-99.5 % identical. In addition, the fifteen strains showed a high degree of nucleotide sequence identity to the early domestic strains (98.4-99.7 %) except the LZC strain, but less sequence identity to the vaccine strain (CV777) used in China (94.7-97.7 %). Phylogenetic analysis showed that the Chinese PEDV strains are composed of a separate cluster including three early domestic strains (JS-2004-02, LJB/03 and DX) but differ genetically from the vaccine strain (CV777) and the early Korean strains (Chinju99 and SM98).

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