Insects have the phenomenon of immune priming by which they can have enhanced protection against reinfection with the same pathogen, and this immune protection can be passed on to their offspring, which is defined as “trans-generational immune priming (TGIP).” But whether housefly possesses TGIP is still unclear. Therefore, we used the housefly as the insect model and Candida albicans as the pathogen to explore whether the housefly is capable of eliciting TGIP, and RNA sequencing (RNA-seq) was performed to explore the molecular mechanism of TGIP of the housefly. We found that the housefly possesses TGIP, and adults pre-exposed to heat-killed C. albicans could confer protection to itself and its offspring upon reinfection with a lethal dose of C. albicans. RNA-seq results showed that 30 and 154 genes were differentially expressed after adults were primed with heat-killed C. albicans (CA-A) and after offspring larvae were challenged with a lethal dose of C. albicans (CA-CA-G), respectively. Among the differentially expressed genes (DEGs), there were 23 immune genes, including 6 pattern recognition receptors (PRRs), 7 immune effectors, and 10 immunoregulatory molecules. More importantly, multiple DEGs were involved in the Toll signaling pathway and phagosome signaling pathway, suggesting that the Toll signaling pathway and phagocytosis might play important roles in the process of TGIP of housefly to C. albicans. Our results expanded on previous studies and provided parameters for exploring the mechanism of TGIP.
Hepatocellular carcinoma (HCC) is a common malignant tumor. However, the mechanisms of the growth, invasion and metastasis of HCC were still unclear. FBXO7 belongs to F-box protein family, which is closely related to tumorigenesis and progression, however, the role of FBXO7 in HCC is still elusive. In this study, we explored the role of FBXO7 in the progression HCC. The expression of FBXO7 in HCC cells was higher than that of normal liver cells. After FBXO7 was silenced by siRNA, the cell proliferation, colony-forming ability, migration and invasion of HCC cells were inhibited. Moreover, the cell cycle of HCC cells was also affected after FBXO7 was silenced, the proportion of cells at G1 phase was increased and the proportion of cells at S phase was decreased. In addition, autophagy of HCC cells was increased after FBXO7 was silenced. In conclusion, FBXO7 may play an oncogenic role in HCC development and progression through autophagy.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.