In this study, we first sequenced and characterized the complete mitochondrial genome of Onychostoma leptura. The complete mitochondrial genome was 16,601 bp in length and included 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), two ribosomal RNA genes (12S rRNA and 16S rRNA), and one non-coding control region (D-loop). The overall nucleotide composition was A: 31.31%, T: 23.89%, G:16.16%, and C: 28.64%, and the A þ T content (55.20%) was much higher than G þ C content (44.80%). The phylogenetic tree indicated that nine species belong to Onychostoma in the present study were classified into two clades. These results can provide basic genetic information for population genetic research of O. leptura and phylogenetic analysis of Onychostoma.
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