Cellular therapies play a critical role in the treatment of spinal cord injury (SCI). Compared with cell-seeded conduits, fully cellular grafts have more similarities with autografts, and thus might result in better regeneration effects. In this study, we fabricated Schwann cell (SC)-neural stem cell (NSC) core–shell alginate hydrogel fibers in a coaxial extrusion manner. The rat SC line RSC96 and mouse NSC line NE-4C were used in this experiment. Fully cellular components were achieved in the core portion and the relative spatial positions of these two cells partially mimic the construction of nerve fibers in vivo. SCs were demonstrated to express more genes of neurotrophic factors in alginate shell. Enhanced proliferation and differentiation tendency of NSCs was observed when they were co-cultured with SCs. This model has strong potential for application in SCI repair.
T-cell immunotherapy holds promise for the treatment of cancer, infection, and autoimmune diseases. Nevertheless, T-cell therapy is limited by low cell expansion efficiency ex vivo and functional deficits. Here we describe two 3D bioprinting systems made by different biomaterials that mimic the in vivo formation of natural lymph vessels and lymph nodes which modulate T-cell with distinct fates and functions. We observe that coaxial alginate fibers promote T-cell expansion, less exhausted and enable CD4+ T-cell differentiation into central memory-like phenotype (Tcm), CD8+ T-cells differentiation into effector memory subsets (Tem), while alginate-gelatin scaffolds bring T-cells into a relatively resting state. Both of the two bioprinting methods are strikingly different from a standard suspension system. The former bioprinting method yields a new system for T-cell therapy and the latter method can be useful for making an immune-chip to elucidate links between immune response and disease.
With the aid of extrusion-based biofabrication strategies, neural stem/progenitor cell-laden hydrogel structures can be fabricated for use in neural research. Extrusion-based strategies can be altered in order to fulfill various requirements. In this study, mouse neural progenitor cell (NE-4C) behaviors in multiple extrusion-based fabricated microenvironments were investigated. Extrusion-based bioprinted cell-laden structures and coaxially extruded core–shell cell fibers were successfully fabricated. Cell distribution and morphology were observed in different structures with scanning electron microscopy (SEM). Genes and proteins related to cell differentiation were examined using quantitative polymerase chain reaction (qPCR) and western blot (WB). The results show that compared with NE-4Cs cultured in petri dishes, the abundance of nestin was 6.28 ± 1.38 times higher in bioprinted structures and the abundances of Tuj-1 and GFAP were 3.14 ± 1.38 and 2.11 ± 0.21 times higher in cell fibers, respectively, indicating that NE-4Cs showed stronger differentiation tendency in cell fibers and weaker tendency in printed structures. This study may provide guidance in selecting fabrication strategies for use in neural research.
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