The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results. Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use. For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS). Experimental results show that these 3D scaffolds exhibit a better swelling ratio because of the embedding of GO. The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells. In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results. It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.
The traditional construction monitoring methods of suspended pole-mounted decomposed towers are mostly manual monitoring. The monitoring personnel has multiple blind spots, and the possibility of misjudgment based on personal experience is relatively large. It is difficult to ensure the construction safety of the suspended pole decomposing tower. For this reason, combined with the current power Internet of Things technology, this paper develops an intelligent monitoring system for suspended pole-mounted decomposing towers. According to the construction technology and its safety requirements of inner suspension derrick for transmission tower erection in sections, this system is classified into intellisense layer, wireless transport layer and information integration layer. According to the physical characteristics of the seven major risk points of the inner suspension pole group tower, the intellisense layer developed corresponding sensing equipment to obtain risk information. In the wireless transport layer, the ZigBee and 4G communication technologies are selected to interconnect self-constituted LAN and 4G wide area networks, to complete on-site data interaction and long-distance transmission. In the information integration layer, the force of cable, the inclination and height of derrick, and the distance between derrick and tower are determined. The system has been verified by the 500 kV delivery project of Fujian Zhouning Pumped Storage Power Station. The average error of critical monitoring point data is 4.14%, and the average data transmission delays in the far and near fields of the system are 18 ms and 176 ms.
Introduction: High-fidelity simulation of the radar echo from the wind turbine (WT) for accurate acquisition of Doppler features, is the key issue in addressing radiation interference from the wind farm on the nearby radar station. In view of the limitation of the conventional scattering center-based equivalent model to reflect the complex surface of blades, it is difficult to simulate the rotating blades’echo accurately with the existing algorithm. Therefore, we proposed a simulation method based on a 3D scattering center extraction to deal with it.Methods: Therefore, we proposed a simulation method based on a 3D scattering center extraction to deal with it. First, the method of scattering center equivalence to blade scattering is used in order to reduce the modelling as well as the solution of electromagnetic scattering from the multi-space attitude of the blade, which is different from the existing algorithm. Since the geometry affects the parameters of the scattering center, an orthogonal matching pursuit greedy algorithm is used to extract the parameter of the 3D scattering center model.Results: Therefore, the temporal correspondence between the scattering center and the blade motion characteristics is established, resulting in a reconstruction of the scattered field data of the rotating blades. Consequently, the real-time simulation and Doppler characteristic of blades echoes are achieved using the Short Time Fourier Transform (STFT).Discussion: A comparison of the results with the data obtained from the GTD scattering center model verififies the accuracy of the proposed method.
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