Background: Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor in adults. The predictive role of SH2D4A has been shown to be closely related to various cancers progression,but there is no comprehensive analysis of the clinical significance in glioblastoma. Hence, this study aimed to explore the relationship between the prognosis of GBM and SH2D4A expression. Methods: The expression of SH2D4A in GBM was analyzed using TIMER2.0 and GEPIA, and validated by qRT-PCR experiments. The CGGA database analyzed the differential expression of SH2D4A in glioma and evaluated the impact of SH2D4A on the survival of glioma patients.LinkedOmics database and GeneMANIA database were studied for SH2D4A co-expression network. A lasso regression model and nomogram were constructed to assess the prognosis of GBM. TCGA database was used to do a GSEA to find functional differences. The relationship between SH2D4A expression and tumor-infiltrating immune cells was analyzed using xCELL, the CIBERSORT algorithm and the TIMER database. Results: In GBM patients, we found that the expression of SH2D4A was upregulated, and the elevated expression of SH2D4A was strongly associated with the grade of the tumor. High SH2D4A expression was found to be a significant independent predictor of poor overall survival (OS) in GBM patients by survival curve analysis and multivariate cox regression analysis. GSEA revealed that SH2D4A was mainly enriched in extracellular matrix tissues, and the expression level of SH2D4A was inversely correlated with the level of infiltration of CD8+T cells, CD4+T cells, B cells, neutrophils and macrophages in GBM, but was positively correlated with the level of dendritic cell infiltration. Immunoassays suggest that altered SH2D4A expression may affect the immune infiltration of GBM tissues and thus affect the survival outcome of GBM. Conclusion: In addition to being a possible prognostic marker and therapeutic target for GBM, SH2D4A may also accelerate the progression of GBM.
Being a third generation advanced nuclear plant type, AP1000 has the characteristics of modular construction, “Open-Top” method lifting, etc, which simultaneously present a new challenge for site HSE management. Through studying Health, Safety & Environment (HSE) site management of the first AP1000 nuclear plant (ANP) under construction, this research analyzes the difference of HSE management aspects, such as management commitment and responsibility, HSE awareness and concept, training and education, HSE reward & punishment system and other aspects between China and the west. It also puts forward the specific idea for developing site HSE management system suitable for ANP technology characteristic and China’s actual conditions, which will provide a guarantee for the safe and smooth construction of ANP project.
Under the background of Sanmen AP1000 NPP Project, World’s First AP1000 Project, this article looks into SPMO management over contractor special process personnel qualification under EPC mode. The project management mode is briefed and the theory basis of the study is discussed, in terms of nuclear safety regulation ASME NQA-1, HAF003 and industrial safety regulations, as well as quality management theory. Contractor special process personnel qualification is generally unmanageable, mainly resulting in negative chain quality problems, objective of the study aims at solving the problems and managerial dilemmas of contractor special process personnel qualification management on site. Quality assurance system is maintained according to ASME NQA-1, and special process is implemented in compliance with the specification of special process control procedures developed for particular special processes, such as welding, NDE, painting, etc. Personnel training, authorization, certificate, operation experiences and pertinent requirements are specified in personnel qualification procedures. The method and process of special process personnel qualification management is created under SPMO quality assurance system, in the scope of SPMO management, while detailed and comprehensive classification of special process personnel qualification is defined with originality. On the basis of ingenious classification and management practice, the author summarizes a systematic management methodology of contractor special process personnel qualification under EPC mode. Classification management, standardized checklist implementation, and site surveillance are integrated for special process personnel management. Outstanding effect and performance are achieved by demonstrating the systematic management methodology, providing effective experience for follow-up AP1000 project which has significant meaning for enhancing contractor special process control and ensuring AP1000 construction quality.
Confined space can be defined as closed or semi-closed equipments, facilities and places which potentially have poor ventilation, poisonous and hazardous gases or poor oxygen. Confined space has complex working environment and more hazardous factors which makes safety accidents occurred frequently. Modularized Construction, as the typical feature of AP1000 nuclear project construction, shortens the construction schedule but increases safety risk, since there is more confined space and cross work, especially in the Containment building after the setting of CVTH. This thesis analyses the confined space construction feature of the containment building and emphasizes the necessity of ventilation and fume control. Some ventilation methods have been taken on site which has effectively reduced the construction risk inside containment buildings. Therefore, this thesis has practical value for the consequent AP1000 project construction.
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