Abstract. Leaf area index is one of the most important parameters in ecological and environmental studies. This paper presents a method for single leaf area measurement based on the counting of the leaf pixels in digital leaf image. Initially, the target leaf is put on a piece of white paper on which four endpoints of an equal-length and orthogonal cross are printed. A background color threshold is then set to separate the white background pixels from the leaf pixels. After the removal of the background pixels, the leaf pixels are left; and the ratio of the number of leaf pixels to the number of pixels of the reference facet formed by the four control endpoints is equal to that of their areas. The single leaf area can then be computed easily based on this proportional relation. Analysis and experimental results indicate that the proposed method is an efficient and precise method for single leaf or leaf-like object area measurement.
Drug discovery has entered a new period of vigorous development with advanced technologies such as DNA-encoded library (DEL) and artificial intelligence (AI). The previous DEL-AI combination has been successfully applied in the drug discovery of classical kinase and receptor targets mainly based on the known scaffold. So far, there is no report of the DEL-AI combination on inhibitors targeting protein-protein interaction, including those undruggable targets with few or unknown active scaffolds. Here, we applied DEL technology on the T cell immunoglobulin and ITIM domain (TIGIT) target, resulting in the unique hit compound 1 (IC50 = 20.7 μM). Based on the screening data from DEL and hit derivatives a1-a34, a machine learning (ML) modeling process was established to address the challenge of poor sample distribution uniformity, which is also frequently encountered in DEL screening on new targets. In the end, the established ML model achieved a satisfactory hit rate of about 75% for derivatives in a high-scored area.
With the rapid development of LBSs (location-based services) in recent years, researchers have increasingly taken an interest in trying to make travel routes more practicable and individualized. Despite the fact that many studies have been conducted on routes using LBS data, the specific routes are deficient in dynamic scalability and the correlations between environmental constraints and personal choices have not been investigated. This paper proposes an improved HMM-based (hidden Markov model) method for planning personalized routes with crowd sourcing spatiotemporal data. It tries to integrate the dynamic public preferences, the individual interests and the physical road network space in the same spatiotemporal framework, ensuring that reasonable routes will be generated. A novel dual-layer mapping structure has been proposed to bridge the gap from brief individual preferences to specific entries of POIs (points-of-interest) inside realistic road networks. A case study on Changsha city has proven that the proposed method can not only flexibly plan people’s travel routes under different spatiotemporal backgrounds but also is close to people’s natural selection by the perception of the group.
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