The distributed fibre sensing technology based on backward stimulated Brillouin scattering (BSBS) is experiencing a rapid development. However, all reported implementations of distributed Brillouin fibre sensors until today are restricted to detecting physical parameters inside the fibre core. On the contrary, forward stimulated Brillouin scattering (FSBS), due to its resonating transverse acoustic waves, is being studied recently to facilitate innovative detections in the fibre surroundings, opening sensing domains that are impossible with BSBS. Nevertheless, due to the co-propagating behaviour of the pump and scattered lights, it is a challenge to position-resolve FSBS information along a fibre. Here we show a distributed FSBS analysis based on recovering the FSBS induced phase change of the propagating light waves. A spatial resolution of 15 m is achieved over a length of 730 m and the local acoustic impedances of water and ethanol in a 30 m-long uncoated fibre segment are measured, agreeing well with the standard values.

The aim of this study is to assess the preventive effects of Lactobacillus fermentum HY01 (LF-HY01) to dextran sulfate sodium induced-colitis. We observed the ratio of colon weight to its length, colon pathological changes, and the concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6) in serum. We also took account of the protein levels of IκBα, NF-κB p65, iNOS, and COX-2, and we measured the best effects of different doses of Lactobacillus fermentum HY01 (low dose group was 109 CFU/kg·bw, high dose group was 1010 CFU/kg·bw) on dextran sulfate sodium-induced colitis mice. The results were remarkable, suggesting that Lactobacillus fermentum HY01 had significant preventive effects in dextran sulfate sodium induced-colitis; simultaneously, the high dose group showed the best results among other groups. It can effectively alleviate the shortened colon length, reduce the ratio of colon weight to its length, reduce edema, inflammatory cells infiltration, and colon mucosa injury, and play an important role in the down-regulation of concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6). Above all, Lactobacillus fermentum HY01 shows promising prevention for IκBα degradation, inhibition of NF-κB p65 phosphorylation cascades, and decreases the protein levels of iNOS and COX-2 as well.

The tumor microenvironment is highly correlated with tumor occurrence, progress, and prognosis. We aimed to investigate the immune-related gene (IRG) expression and immune infiltration pattern in the tumor microenvironment of lower-grade glioma (LGG). We employed the Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) algorithm to calculate immune and stromal scores and identify prognostic IRG based on The Cancer Genome Atlas data set. The potential molecular functions of these genes were explored with the help of functional enrichment analysis and the protein-protein interaction network. Remarkably, three cohorts that were downloaded from the Chinese Glioma Genome Atlas database were analyzed to further verify the prognostic values of these genes. Moreover, the Tumor IMmune Estimation Resource (TIMER) algorithm was used to estimate the abundance of infiltrating immune cells and explore the immune infiltration pattern in LGG. And unsupervised cluster analysis determined three clusters of the immune infiltration pattern and indicated that CD8 + T cells and macrophages were significantly associated with LGG outcomes. Altogether, our study identified a list of prognostic IRGs and provided a perspective to explore the immune infiltration pattern in LGG.

Systematic errors induced by distortions in the pump pulse of conventional Brillouin distributed fiber sensors are thoroughly investigated. Experimental results, supported by a theoretical analysis, demonstrate that the two probe sidebands in standard Brillouin optical time-domain analyzers provide a non-zero net gain on the pump pulse, inducing severe distortions of the pump when scanning the pump-probe frequency offset, especially at high probe power levels. Compared to the impact of non-local effects reported in the state-of-the-art, measurements here indicate that for probe powers in the mW range (below the onset of amplified spontaneous Brillouin scattering), the obtained gain and loss spectra show two strong side-lobes that lead to significant strain/temperature errors. This phenomenon is not related to the well-known spectral hole burning resulting from pump depletion, but it is strictly related to the temporal and spectral distortions that the pump pulse experiences when scanning the Brillouin gain/loss spectrum. As a solution to this problem, a novel scanning scheme for Brillouin sensing is proposed. The method relies on a fixed frequency separation between the two probe sidebands, so that a flat zero net gain is achieved on the pump pulse when scanning the pump-probe frequency offset. The proposed technique is experimentally validated, demonstrating its ability to completely cancel out non-local effects up to a probe power ultimately limited by the onset of amplified spontaneous Brillouin scattering. The method allows for one order of magnitude improvement in the figure-of-merit of optimized long-range Brillouin distributed fiber sensors, enabling measurements along a 100 km-long sensing fiber with 2 m spatial resolution and with no need of added features for performance enhancement. References and links 1. T. Horiguchi, K. Shimizu, T. Kurashima, M. Tateda, and Y. Koyamada, "Development of a distributed sensing technique using Brillouin scattering," J. Lightwave Technol. 13(7), 1296-1302 (1995

Distributed optical fibre sensors deliver a map of a physical quantity along an optical fibre, providing a unique solution for health monitoring of targeted structures. Considerable developments over recent years have pushed conventional distributed sensors towards their ultimate performance, while any significant improvement demands a substantial hardware overhead. Here, a technique is proposed, encoding the interrogating light signal by a single-sequence aperiodic code and spatially resolving the fibre information through a fast post-processing. The code sequence is once forever computed by a specifically developed genetic algorithm, enabling a performance enhancement using an unmodified conventional configuration for the sensor. The proposed approach is experimentally demonstrated in Brillouin and Raman based sensors, both outperforming the state-of-the-art. This methodological breakthrough can be readily implemented in existing instruments by only modifying the software, offering a simple and cost-effective upgrade towards higher performance for distributed fibre sensing.

A mutation in the isocitrate dehydrogenase 1 (IDH1) gene is the most common mutation in diffuse lower-grade gliomas (LGGs), and it is significantly related to the prognosis of LGGs. We aimed to explore the influence of the IDH1 mutation on the immune microenvironment and develop an IDH1-associated immune prognostic signature (IPS) for predicting prognosis in LGGs. IDH1 mutation status and RNA expression were investigated in two different public cohorts. To develop an IPS, LASSO Cox analysis was conducted for immune-related genes that were differentially expressed between IDH1wt and IDH1mut LGG patients. Then, we systematically analyzed the influence of the IPS on the immune microenvironment. A total of 41 immune prognostic genes were identified based on the IDH1 mutation status. A four-gene IPS was established and LGG patients were effectively stratified into low- and high-risk groups in both the training and validation sets. Stratification analysis and multivariate Cox analysis revealed that the IPS was an independent prognostic factor. We also found that high-risk LGG patients had higher levels of infiltrating B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages and dendritic cells, and expressed higher levels of CTLA-4, PD-1 and TIM-3. Moreover, a novel nomogram model was established to estimate the overall survival in LGG patients. The current study provides novel insights into the LGG immune microenvironment and potential immunotherapies. The proposed IPS is a clinically promising biomarker that can be used to classify LGG patients into subgroups with distinct outcomes and immunophenotypes, with the potential to facilitate individualized management and improve prognosis.

Abstract:The robustness of bipolar pulse coding against pump depletion issues in Brillouin distributed fiber sensors is theoretically and experimentally investigated. The presented analysis points out that the effectiveness of bipolar coding in Brillouin sensing can be highly affected by the power unbalance between -1's and + 1's elements resulting from depletion and amplification of coded pump pulses. In order to increase robustness against those detrimental effects and to alleviate the probe power limitation imposed by pump depletion, a technique using a three-tone probe is proposed. Experimental results demonstrate that this method allows increasing the probe power by more than 12.5 dB when compared to the existing single-probe tone implementation. This huge power increment, together with the 13.5 dB signal-to-noise enhancement provided by 512-bit bipolar Golay codes, has led to low-uncertainty measurements (< 0.9 MHz) of the local Brillouin peak gain frequency over a real remoteness of 100 km, using a 200 km-long fiber-loop and 2 m spatial resolution. The method is evaluated with a record figure-of-merit of 380'000. Gonzalez-Herraez, and L. Thévenaz, "Extending the real remoteness of long-range Brillouin optical time-domain fiber analyzers," J. Lightwave Technol. 32(1), 152-162 (2014). 18. Y. Dong, L. Chen, and X. Bao, "Extending the sensing range of Brillouin optical time-domain analysis combining frequency-division multiplexing and in-line EDFAs," J.

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