Long non-coding RNAs (lncRNAs) played vital roles in
physiological
and pathological conditions. Consistent results from cell experiments,
animal experiments, and clinical studies suggested that lncRNA HULC
was an oncogenic lncRNA serving as a potential diagnostic and prognostic
marker of hepatocellular carcinoma. In this study, we developed a
fluorescent biosensor for lncRNA HULC detection based on rolling circle
amplification (RCA) induced by multi-primer probes. Multiple primer
probes can not only combine with lncRNA to break its secondary structure,
which was conducive to lncRNA captured by Y-shaped probes, but also
trigger multiple RCA reactions to achieve signal amplification and
the goal of sensitive detection of lncRNA. Compared to previous detection
methods, in this scheme, we took advantage of the long sequence characteristics
of lncRNA to make it a carrier that can bind multiple primers to initiate
RCA. This newly designed biosensor provided a linear range from 1
pM to 100 nM with a detection limit of 0.06 pM. This method can provide
a new idea for the application of isothermal amplification in detecting
lncRNA. Furthermore, the application of the biosensor in liver cancer
cell lines and whole blood samples from hepatocellular carcinomatosis
patients also confirmed that the method had good selectivity and sensitivity
to lncRNA HULC. This method offered a new way for transforming specific
lncRNA into clinical application for diagnosis, prognosis, or predicting
treatment response.
Heteromultivalent scaffolds with different repeated monomers have great potential in biomedicine, but convenient construction strategies for integrating various functional modules to achieve multiple biological functions are still lacking. Here, taking...
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