Pulmonary
fibrosis (PF) is a kind of interstitial lung disease
with the features of progressive and often fatal dyspnea. Tetrandrine
(TET) is the major active constituent of Chinese herbal Stephania
tetrandra S. Moore, which has already applied clinically
to treat rheumatism, lung cancer, and silicosis. In this work, a tetrandrine-hydroxypropyl-β-cyclodextrin
inclusion compound (TET-HP-β-CD) was developed for the treatment
of pulmonary fibrosis via inhalation administration. TET-HP-β-CD
was prepared by the freeze-drying method and identified using the
cascade impactor, differential scanning calorimetry (DSC), X-ray diffraction
(XRD), and Fourier transform infrared spectrum (FT-IR). A bleomycin-induced
pulmonary fibrosis rat model was used to assess the effects of inhaled
TET and TET-HP-β-CD. Animal survival, hydroxyproline content
in the lungs, and lung histology were detected. The results showed
that inhalation of TET-HP-β-CD alleviated inflammation and fibrosis,
limited the accumulation of hydroxyproline in the lungs, regulated
protein expression in PF development, and improved postoperative survival.
Moreover, nebulized delivery of TET-HP-β-CD accumulated chiefly
in the lungs and limited systemic distribution compared with intravenous
administration. The present results indicated that inhalation of TET-HP-β-CD
is an attractive candidate for the treatment of pulmonary fibrosis.
Silybin is a flavonoid lignin compound consisting of two diastereomers with nearly equal molar ratios. It has been reported that silybin can effectively inhibit the aggregation of amyloid protein, but the difference between the two silybin diastereomers has been rarely studied. In this work, the inhibitory ability of silybin to hen egg-white lysozyme (HEWL) was demonstrated, and the difference of kinetic parameters of two diastereomers was analyzed. Fluorescence quenching titration was utilized to analyze the binding differences to native HEWL between the diastereomers, and transmission electron microscopy (TEM) was utilized to analyze the characteristics of the surface of various samples. The differences between hydrophobicity and the secondary structure among several HEWL samples were measured by the 8-anilino-1-naphthalene sulfonic (ANS) acid fluorescence probe, Raman spectra, and far-UV circular dichroism. Moreover, the differences in the binding energy of these two diastereomers with HEWL were analyzed by molecular docking. Also, we have investigated the effect of silybin diastereomers on HEWL fibril-induced cytotoxicity in SH-SY5Y cells. Results show that silybin has a certain inhibitory effect on the HEWL fibrillogenesis process, while silybin B (SB) has a more significant inhibitory effect than silybin A (SA), especially at high concentrations. This work provides some insights into the screening of amyloid inhibitors from complicated natural products and indicates that SB has the prospect of further development as an amyloid inhibitor.
Resistance and tolerance of biofilms to antibiotics is the greatest challenge in the treatment of bacterial infections. Therefore, developing an effective strategy against biofilms is a top priority. Liposomes are widely used as antibiotic drug carriers; however, common liposomes lack affinity for biofilms. Herein, biofilm-targeted antibiotic liposomes are created by simply adjusting their cholesterol content. The tailored liposomes exhibit significantly enhanced bacterial inhibition and biofilm eradication effects that are positively correlated with the cholesterol content of liposomes. The experiments further demonstrate that this enhanced effect can be ascribed to the effective drug release through the pores, which are formed by the combination of cholesterol microdomains in liposomal lipid bilayers with membrane-damaged toxins in biofilms. Consequently, liposome encapsulation with a high cholesterol concentration improves noticeably the pharmacodynamics and biocompatibility of antibiotics after pulmonary administration. This work may provide a new direction for the development of antibiofilm formulations that can be widely used for the treatment of infections caused by bacterial biofilms.
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