Objective. Statin treatment alone has been demonstrated to significantly increase plasma proprotein convertase subtilisin/kexin type 9 (PCSK9) levels. The effect of policosanol combined with statin on PCSK9 is unknown. Methods. Protocol I: 26 patients with atherosclerosis were randomly assigned to receive either atorvastatin 20 mg/d or policosanol 20 mg/d + atorvastatin 20 mg/d for 8 weeks. Protocol II: 15 healthy volunteers were randomly assigned to either policosanol 20 mg/d or a control group for 12 weeks. Serum levels of PCSK9 were determined at day 0 and the end of each protocol. Results. Protocol I: atorvastatin 20 mg/d significantly increased serum PCSK9 level by 39.4% (256 ± 84 ng/mL versus 357 ± 101 ng/mL, P = 0.002). However, policosanol 20 mg/d + atorvastatin 20 mg/d increased serum PCSK9 level by only 17.4% without statistical significance (264 ± 60 ng/mL versus 310 ± 86 ng/mL, P = 0.184). Protocol II: there was a trend toward decreasing serum PCSK9 levels in the policosanol group (289 ± 71 ng/mL versus 235 ± 46 ng/mL, P = 0.069). Conclusion. Policosanol combined with statin attenuated the statin-induced increase in serum PCSK9 levels. This finding indicates that policosanol might have a modest effect of lowering serum PCSK9 levels.
From the fruits of Kochia scoparia (L.) Schrad, five triterpenoid glycosides were isolated for the first time from this plant. They were elucidated as momordin Ic, the 6'-methyl ester of momordin Ic, momordin IIc, 2'-O-beta-D-glucopyranosylmomordin Ic, and 2'-O-beta-D-glucopyranosylmomordin IIc on the basis of spectral and chemical methods. The last two saponins are new natural products.
In weak acid medium, aluminum(III) can react with chlorophosphonazo III [CPA(III), H(8)L] to form a 1:1 coordination anion [Al(OH)(H(4)L)](2-). At the same time, proteins such as bovine serum albumin (BSA), lysozyme (Lyso) and human serum albumin (HSA) existed as large cations with positive charges, which further combined with [Al(OH)(H(4)L)](2-) to form a 1:4 chelate. This resulted in significant enhancement of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS). In this study, we investigated the interaction between [Al(OH)(H(4)L)](2-) and proteins, optimization of the reaction conditions and the spectral characteristics of RRS, SOS and FDS. The maximum RRS wavelengths of different protein systems were located at 357-370 nm. The maximum SOS and FDS wavelengths were located at 546 and 389 nm, respectively. The scattering intensities (ΔI) of the three methods were proportional to the concentration of the proteins, within certain ranges, and the detection limits of the most sensitive RRS method were 2.6-9.3 ng/mL. Moreover, the chelate reaction mechanism or the reasons for the enhancement of RRS were discussed through absorption spectra, fluorescence spectra and circular dichroism (CD) spectra.
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