The inner mitochondrial membrane (IMM) invaginates to form cristae and the maintenance of cristae depends on the mitochondrial contact site (MICOS) complex. Mitofilin and CHCHD6, which physically interact, are two components of the MICOS. In this study, we performed immunoprecipitation experiments with Mitofilin and CHCHD6 antibodies and identified a complex containing Mitofilin, Sam50, and CHCHD 3 and 6. Using transcription activator-like effector nucleases (TALENs), we generated knockdown/knockout clones of Mitofilin and CHCHD6. Transmission electron microscopy (TEM) revealed that vesicle-like cristae morphology appeared in cell lines lacking Mitofilin, and mitochondria exhibited lower cristae density in CHCHD6-knockout cells. Immunoblot analysis showed that knockdown of Mitofilin, but not knockout of CHCHD6, affected their binding partners that control cristae morphology. We also demonstrated that Mitofilin and CHCHD6 directly interacted with Sam50. Additionally, we observed that Mitofilin-knockdown cells showed decreased mitochondrial membrane potential (ΔΨm) and intracellular ATP content, which were minimally affected in CHCHD6-knockout cells. Taken together, we conclude that the integrity of MICOS and its efficient interaction with Sam50 are indispensable for cristae organization, which is relevant to mitochondrial function.
Cycle-to-cycle variations during the combustion phase should be comprehensively investigated because these variations are among the most serious causes of higher emissions and lower efficiency. The main objective of this study was to evaluate the relationship between cyclic variations and combustion parameters. The combustion and cyclic variation characteristics were investigated using a diesel engine operating on Fischer–Tropsch (F–T) fuel synthesized from coal. Experiments were conducted under full load conditions at three engine speeds of 1200, 2000, and 2800 rpm. The results revealed that cyclic variations of F–T diesel were lower than those of 0# diesel, acquired the minimum value at the speed of 2000 rpm, and reached the maximum at the speed of 2800 rpm. The mean fluctuation intensity of F–T diesel was 0.185, 0.189, and 0.205 at speeds of 1200, 2000, and 2800 rpm, respectively, smaller than that of 0# diesel under the corresponding conditions. The relationships between cyclic variations and combustion parameters were analyzed by correlation methods. Maximum in-cylinder pressure (Pmax) increased linearly with increased ignition delay, while it decreased linearly with increased combustion duration. The Pearson’s correlations between Pmax and ignition delay were 0.75, 0.78, and 0.73; however, the corresponding values between Pmax and combustion duration were 0.61, 067, and 0.65 when fueled with F–T diesel at speeds of 1200, 2000, and 2800 rpm, respectively. Moreover, the Pearson’s correlations of 0# diesel were higher than those of F–T diesel at the same operating loads. Compared with combustion duration, the ignition delay had more important effects on cyclic variations with a higher Pearson’s correlation. Furthermore, the ignition delay significantly influenced cyclic variation under a high speed load, while the combustion duration had a marked effect under low speed conditions. Overall, the results revealed the importance of combustion parameters on cyclic variation, which has great significance for controlled cyclic variation in diesel engines.
This article intends a hybrid energy harvesting shock absorber design which comprehends energy harvesting of automobile suspension vibration dissipation. A mathematical model of the energy harvesting prototype is established, and simulation results show that the dissipation energy can be recovered by varying the feed module, thereby got the damping forces ratio at different compression and extension stroke. The energy conversion from hydraulic energy to mechanical energy mainly then mechanical energy converted into electrical energy furthermore we can rechange our battery from this recovered energy. The advanced mathematical model and prototype proposed maximum ride comfort meanwhile recovered the suspension energy and fuel saving. This article shows the simulation results verifying it with prototype test results. The damping force of expansion stroke is higher than the damping force of compression stroke. The damping characteristics curves and speed characteristics curves verify the validity by simulation and prototyping damper at different amplitudes of off-road vehicles. The Hydraulic Electromagnetic Regenerative Shock Absorber (HESA) prototype characteristic is tested in which 65 watts recovered energy at 1.67Hz excitation frequency. So, 14.65% maximum energy recovery efficiency got at 20mm rod diameter and 8 cc/rev motor displacement. The damping characteristics of the HESA prototype examined and it has ideal performance as the standard requirements of the National Standard QC/T 491-1999.
Abstract-Condition monitoring (CM) of gearbox is a crucial activity due to its importance in power transmission for many industrial applications. Monitoring temperature is an effective mean to collect useful information about the healthy conditions of the gearbox. This study investigates the use of a novel wireless temperature node to monitor and diagnose different faults on a gearbox transmission system under different conditions. The wireless temperature node was fabricated with a novel feature that it is supplied by a thermoelectric generator module mounted on the gearbox house to be monitored so that the measurement system avoids the shortage of using a wired power sources or the requirement for recharging or changing batteries. Moreover, the temperatures from lubricating oils and housing are modelled empirically to implement a model based detection. The results show that this monitoring approach allows a number of common faults: tooth breakage, oil shortage, and shaft misalignment to be separated under different loads, which demonstrates the outstanding performance of the proposed system and thus suitable for online and automated condition monitoring.
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