Soil microbial community very little had been found as trace evidence, however, it could be vital for forensic science. In this paper, soil microbial community diversities from seventeen different regions of Shandong province were investigated by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The results of diversity index analysis show the highest Margalef index and Eveness index are 4.609 for sample D and 0.970 for sample E, respectively. In T-RFLP profile, the absolute advantage peak are 488bp (62.6% ) from sample O and 496.5bp (20.8%) from Sample H. The analysed terminal restriction fragments (T-RFs) are from Firmicutes and Proteobacteria. These results reveal that there are the obvious regional characteristic for microbial community of all the detected samples, which suggests T-RFLP analysis of microbial community diversity will be a rapid effective fingerprinting method for forensic detection.
The basic requirements for low-carbon building are the natural life such as natural light, ventilation, climbing the stairs and so on. For example, well-designed natural ventilation needs no energy and cleaner than the mechanical ventilation, so the natural ventilation has a much better effect. The space organization should prevent the people to fall into the depraved life of higher energy consumption. Actually, saving water and electricity in life provide the better effect than the expensive saving water and electricity system. This kind of building need no complex pipe laying and much cost of maintenance and management after it is used.
Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. It is a high-molecular-weight polymer consisting of repeating octasaccharide units. These units are synthesized by a complex pathway encoded by numbers of exo genes. In this study, two key genes,exoAandexoY, were cloned and sequenced, which controlled the first two glycosyltransferase reactions in the biosynthesis of succinoglycan. The sequences contained 999-base-pares (bp) and 681-bp Open reading Frame (ORF) encoding 332 and 236 amino-acid proteins with molecular weights of approximate 36.8 kDa and 25.5 kDa , respectively. The putative proteins, ExoA and ExoY, were analyzed by several online protein analysis softwares. The results showed ExoA and ExoY were the membrane proteins with three (ExoA) and one (ExoY) hydrophobic transmembrance domains. Their theoretical PI values were 9.49 and 9.34, respectively. The second structures analysis indicated that they were composed of 45.48% and 38.94% α-helix, 13.55% and 16.81% β-sheet, and 40.96% and 44.25% random coil structures respectively. These data will lay a foundation for the subsequent 3D structure prediction and gene mutation to improve succinoglycan production.
A β-(1,3)-D-glucan (curdlan) synthase gene for a low molecular weight curdlan biosynthesis, crdSAg, from Agrobacterium sp. M503 was cloned and its encoding protein was characterized by several online protein analysis softwares. The crdSAg consists of 1965-base-pairs Open Reading Frame (ORF) encoding a protein with molecular weight approximate 73.5 kDa, which contains the conserved domain of CESA-CelA_like belonging to glycosyltransferase family 2 (GT2). Moreover, CrdSAg was a membrane protein with seven hydrophobic transmembrance domains. The second structure analysis indicated it was composed of 43.12% α-helix, 17.89% β-sheet, and 38.99% random coil structure. These data will lay a foundation to clarify the biosynthesis mechanism of the low molecular weight curdlan.
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