Apoptosis is a form of cell death that involves the changes of mitochondrial function and the regulated activation of caspase cascades, which selectively cleave cytoskeleton proteins and catalyze the changes of cell organelles and morphological structure. The changes of mitochondrial function, cell morphological structure, and degradation of cytoskeleton are considered to be responsible for the development of meat qualities. The LM, semitendinosus, and psoas minor (PM) muscles of 5 crossbred bulls were used to observe the morphologic and quantitative changes of apoptosis, as well as the change of caspase-3 activity during 7 d storage. Transmission electron microscopy shows that the typical features of apoptosis appeared in muscles between d 1 and 4. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL) positive nuclei were detected at d 4 and increased subsequently. The count of TUNEL-positive nuclei was different in 3 muscles at d 7 (P < 0.001). There was a significant increase in caspase-3 activities at 4 h postmortem relative to the activities at the first 30 min in 3 muscles (P = 0.0147 in LM; P = 0.0058 in PM; P = 0.0306 in semitendinosus), and the apexes had 2.9 to 6.5 times more activities than activities at the first 30 min postmortem. Apoptosis did exist in 3 types of muscles during the conditioning period. Apoptosis and caspase cascades system could be associated with the postmortem development of meat quality in skeletal muscles of bulls.
NASICON‐type sodium vanadium phosphate (Na3V2(PO4)3, or NVP) cathode materials have great potential for fast charging and long cycling sodium‐ion batteries (SIBs) similar to lithium iron phosphate (LiFePO4, or LFP) cathode materials used in lithium‐ion batteries (LIBs). However, the cycle life and energy density in the full cell using NVP materials need to be significantly improved. This paper investigates the degradation mechanisms of NVP‐based SIBs and identifies the Na loss from the cathode to the anode solid electrolyte interphase (SEI) reactions as the main cause of capacity degradation. A new Na‐rich NVP cathode (e.g., Na4V2(PO4)3, or Na4VP) is developed to address the Na loss problem. Conventional NVP can be easily transformed into the Na4VP by a facile and fast chemical solution treatment (30 s). Na‐free‐anode Na4VP and hard carbon‐Na4VP full cells are assembled to evaluate the electrochemical properties of the Na‐rich NVP cathode. The Na4VP cathode provides excess Na to compensate for the Na loss, resulting much longer cycle life in the full cells (>400 cycles) and a high specific energy and power density. Good low‐temperature performance is also observed.
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