Recombinant adeno-associated virus (AAV) vectors are promising tools for gene therapy. In spinal cord injury where extensive damage occurs, vectors with high diffusion and transduction abilities are required. We compared the diffusion capacity and transduction efficiency of AAV2 and AAV5 vectors using a mouse spinal cord injury model. Our study demonstrates that AAV5 is more effective than AAV2 for delivering genes into the injured spinal cord tissue. AAV5 diffused 6.9 mm from the injection site, transduced with an approximately two-fold increase in total cell number and yielded an approximately three-fold increase in gene expression in comparison with AAV2.
Abstract. Crankshaft assembly, as one of the most important parts of the engine, always suffers many kinds of cyclic loading during service. Due to the higher requirements for dynamic property of mechanical system, it is necessary to work on multi-body dynamics simulation of crankshaft assembly quick and accurately. The multi-body dynamics simulation analysis was carried out to the crankshaft assembly in V-type 12-cylinder large diesel by dynamics analysis software ADAMS in this work. The kinematic characteristics of piston and load characteristic of connecting rod are obtained via simulations. The comparison of loads on connecting rod between simulation result and analytical calculation result verified the accuracy of multi-body dynamics simulation of crankshaft assembly based on ADAMS. The simulation results can provided basis for finite element analysis of crankshaft and provided guidance for improving the dynamic characteristic of crankshaft assembly.
Abstract. Modal performance of the automotive instrument panel has been studied in this article. Finite element software Hypersh/ OptiStruct has been used in modal analysis in this article. This paper first calculated the modal frequency and vibration shape of the instrument panel. Engineered modification has been applied to rise the inherent frequency. The result shows this method can effectively optimize the inherent frequency of the instrument panel assembly.
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