Cholestatic liver injury, characterized by liver fibrosis, has increasingly become a global health problem, with no effective treatment available. Hepatic stellate cells (HSCs) differentiate into myofibroblasts, leading to excessive deposition of the extracellular matrix (ECM), which is a feature of liver fibrosis. Basic fibroblast growth factor (bFGF) has proven antifibrotic effects in chronic liver disease; however, the lack of an effective delivery system to the injury site reduces its therapeutic efficacy. The aim of this study was to assess the therapeutic effect of collagen-binding bFGF (CBD-bFGF) for the treatment of liver fibrosis in a murine bile duct ligation (BDL) model. We found that CBD-bFGF treatment significantly alleviated liver injury in the early phase of BDL injury, and was associated with decreased necroptotic cell death and inflammatory response. Moreover, CBD-bFGF had enhanced therapeutic effects for liver fibrosis on day 7 after surgery compared to those obtained with native bFGF treatment. In vitro, CBD-bFGF treatment notably inhibited TGF-β1-induced LX-2 cell activation, migration, and contraction compared with native bFGF. In conclusion, CBD-bFGF may be a promising treatment for hepatic fibrosis.
It uses MSP430F2274, the ultra low power microcontroller, as the control core of short circuit detection device, through the accurate measurement of low voltage circuit breaker load side of the main wiring of insulation resistance value and the interphase insulation resistance value, outputting high voltage startup sound and light alarm device, and then accomplish the open circuit fault detection. Testing power supply is the use of magnetic coupling resonance principle, from the main power grid electrical insulation for short circuit detection device. The device has the advantages of low cost, small volume, good safety, good detection effect, and can be embedded in use in low voltage circuit breaker.
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