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To simultaneously determine three components of aconitine, mesaconitine, and hypaconitine in six species of Aconitum genus, an extraction condition for the total alkaloids was specifically optimized and a simple analytical method of reversed-phased highperformance liquid chromatography (HPLC) was developed. The extraction rate of total alkaloids in A. szechenyianum Gay was 98.3% for repeated extracting three times with an acidic alcohol solution (alcohol: pH 3.0 HAc = 85:15, v/v). The chromatography was carried out on a Phenomenex Luna C(18) column by gradient elution with a mobile phase of 0.03 mol/mL ammonium bicarbonate (pH = 9.50) -acetonitrile at a flow rate of 1.0 mL/min. The method for all three alkaloids had good linear relationships (r > 0.999) in the concentration range of 1.0-200.0 μg/mL. The average recoveries were 96.6-103.1%, and the LOQ and LOD were in the range of 25-37 ng/mL and 9-12 ng/mL, respectively. The quantitative results indicated that contents of the three alkaloids varied significantly among crude aconite roots, so quality control of traditional Chinese medicines containing aconite roots should be taken into account.

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Two napelline skeletal diterpenoid alkaloids 15-acetylsongorine, C 24 H 33 NO 4 I, and songoramine, C 22 H 29 NO 3 II, were first isolated from the roots of Aconitum Szechenyianum Gay. The crystal structures were determined by X-ray single-crystal diffraction analysis. The crystal I is the triclinic system with space group P1 having unit cell parameters of a = 9.360(8) Å , b = 11.593(9) Å , c = 11.830 (16) Å , a = 113.223(15)°, b = 105.950(16)°, c = 101.296(12)°, and Z = 2. Hydrogen bonds O-HÁÁÁO and O-HÁÁÁN joint the molecules into dimer. The crystal II belongs to the orthorhombic system with space group P2 1 2 1 2 1 having unit cell parameters of a = 8.950(2) Å , b = 13.272(3) Å , c = 15.454(4) Å and Z = 4. The O-HÁÁÁO hydrogen bonding interaction links the molecule into linear chains. The distortion of rings of compound I and II were evaluated by calculation of the Cremer and Pople puckering parameters. The presence of the C-O-C bond in the compound II results in the changes of ring conformations compared with that of the compound I.

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