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Bacillus subtilis spore preparations are promising probiotics and biocontrol agents, which can be used in plants, animals, and humans. The aim of this work was to optimize the nutritional conditions using a statistical approach for the production of B. subtilis (WHK-Z12) spores. Our preliminary experiments show that corn starch, corn flour, and wheat bran were the best carbon sources. Using Plackett-Burman design, corn steep liquor, soybean flour, and yeast extract were found to be the best nitrogen source ingredients for enhancing spore production and were studied for further optimization using central composite design. The key medium components in our optimization medium were 16.18 g/l of corn steep liquor, 17.53 g/l of soybean flour, and 8.14 g/l of yeast extract. The improved medium produced spores as high as 1.52 +/- 0.06 x 10(10) spores/ml under flask cultivation conditions, and 1.56 +/- 0.07 x 10(10) spores/ml could be achieved in a 30-l fermenter after 40 h of cultivation. To the best of our knowledge, these results compared favorably to the documented spore yields produced by B. subtilis strains.

Miami, Florida USA S K m VThe Gompertz distribution has been used to model human mortality and fit actuarial tables. In the recent years, this distribution has been studied by some authors. The MLE estimates for the parameters of the Gompertz distribution we= disucssed by GmG. RAO and REDMOM) (1970). The purpose of this paper is to develop an exact confidence interval and an exact joint confidence region for the parameters of the Gompertz distribution.

In the last decade, silicon photonic switches are increasingly believed to be potential candidates for replacing the electrical switches in the applications of telecommunication networks, data center and high-throughput computing, due to their low power consumption (Picojoules per bit), large bandwidth (Terabits per second) and high-level integration (Square millimeters per port). This review paper focuses on the state of the art and our perspectives on silicon photonic switching technologies. It starts with a review of three types of fundamental switch engines, i.e., Mach-Zehnder interferometer, micro-ring resonator and micro-electro-mechanical-system actuated waveguide coupler. The working mechanisms are introduced and the key specifications such as insertion loss, crosstalk, switching time, footprint and power consumption are evaluated. Then it is followed by the discussion on the prototype of large-scale silicon photonic fabrics, which are based on the configuration of above-mentioned switch engines. In addition, the key technologies, such as topological architecture, passive components and optoelectronic packaging, to improve the overall performance are summarized. Finally, the critical challenges that might hamper the silicon photonic switching technologies transferring from proof-of-concept in lab to commercialization are also discussed.

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A study was undertaken to develop a methodology to estimate annual average daily traffic (AADT) for nonstate roads in urbanized areas in Florida. The current practice related to the estimation of AADT for nonstate roads has been of great concern to the Florida Department of Transportation because of the potential lack of accuracy in the estimated data. For this study, a multiple regression model was developed for estimating AADT on nonstate roads. The model utilized a large sample size (data from 450 count stations in Broward County) and involved the investigation of up to 12 initial variables. Various methods, including geographic information systems (GIS), were explored to convert the current digital data and aggregate them into suitable forms for statistical analysis. Statistical tests were performed and the results showed that the most important contributing predictors are roadway characteristics, such as the number of lanes, functional classification, and area type. Socioeconomic variables, including population, dwelling units, automobile ownership, employment numbers, and school enrollment in the surrounding area, have an insignificant impact on AADT. Further analyses revealed the deficiency of traditional roadway functional classification, the need to improve the method of road classification, and the need for alternative methods to account for the impact of economic activities on AADT.

BackgroundBacillus subtilis is widely used in agriculture and industrial biotechnology; however, cell autolysis significantly decreases its yield in liquid cultures. Numerous factors mediate the lysis of B. subtilis, such as cannibalism factors, prophages, and peptidoglycan (PG) hydrolases. The aim of this work was to use molecular genetic techniques to develop a new strategy to prevent cell lysis and enhance biomass as well as the production of recombinant proteins.ResultsFive genes or genetic elements representing three different functional categories were studied as follows: lytC encoding PG hydrolases, the prophage genes xpf and yqxG-yqxH-cwlA (yGlA), and skfA and sdpC that encode cannibalism factors. Cell lysis was reduced and biomass was enhanced by deleting individually skfA, sdpC, xpf, and lytC. We constructed the multiple deletion mutant LM2531 (skfA sdpC lytC xpf) and found that after 4 h of culture, its biomass yield was significantly increased compared with that of prototypical B. subtilis 168 (wild-type) strain and that 15% and 92% of the cells were lysed in cultures of LM2531 and wild-type, respectively. Moreover, two expression vectors were constructed for producing recombinant proteins (β-galactosidase and nattokinase) under the control of the P43 promoter. Cultures of LM2531 and wild-type transformants produced 13741 U/ml and 7991 U/ml of intracellular β-galactosidase, respectively (1.72-fold increase). Further, the level of secreted nattokinase produced by strain LM2531 increased by 2.6-fold compared with wild-type (5226 IU/ml vs. 2028 IU/ml, respectively).ConclusionsOur novel, systematic multigene deletion approach designed to inhibit cell lysis significantly increased the biomass yield and the production of recombinant proteins by B. subtilis. These findings show promise for guiding efforts to manipulate the genomes of other B. subtilis strains that are used for industrial purposes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-014-0129-9) contains supplementary material, which is available to authorized users.

In this paper a new sensing scheme by simultaneously measuring optical refractive index change and sound speed change in an optofluidic thin wall micro-bubble resonator is reported. Sensitivity of sound speed is 4.2-6.8 MHz/ (km/s) for 3 types of mechanical modes. A 2-D optical/opto-mechanical sensing map is plotted by detecting both the whispering gallery mode resonance shift and the optomechanical resonance shift. This novel scheme provides a supplementary support to optical sensing when analytes do not respond to refractive index (RI) change.