Graphene materials have unique structures and outstanding thermal, optical, mechanical and electronic properties. In the last decade, these materials have attracted substantial interest in the field of nanomaterials, with applications ranging from biosensors to biomedicine. Among these applications, great advances have been made in the field of antibacterial agents. Here, recent advancements in the use of graphene and its derivatives as antibacterial agents are reviewed. Graphene is used in three forms: the pristine form; mixed with other antibacterial agents, such as Ag and chitosan; or with a base material, such as poly (N-vinylcarbazole) (PVK) and poly (lactic acid) (PLA). The main mechanisms proposed to explain the antibacterial behaviors of graphene and its derivatives are the membrane stress hypothesis, the oxidative stress hypothesis, the entrapment hypothesis, the electron transfer hypothesis and the photothermal hypothesis. This review describes contributions to improving these promising materials for antibacterial applications.

A methodology that takes into account the (n,m) structure of single-walled carbon nanotubes (SWNTs), through an exciton-exciton resonance model and an electron-phonon interaction model, was employed in order to evaluate the semiconducting (n,m) abundance of two SWNT samples (i.e., Co-MCM-41 and HiPco). This was based on photoluminescence and near-infrared absorption data obtained on aqueous suspensions of individually dispersed SWNTs. In the absence of known (n,m) abundance SWNT samples, we resorted to determining the diameter distribution curves for both samples, which were found to obey an unsymmetrical log-normal distribution, typical for vapor-phase particle growth. Using this log-normal function, we reconstructed the near-infrared E S11 absorption spectrum of the narrow diameter distribution Co-MCM-41 SWNT sample, which in turn enabled us to assess the predictions of these two theoretical models. High spectral reconstruction accuracy was obtained from the electron-phonon interaction model when considering (11,0) and (10,0) zigzag nanotubes, along with (n,m) line widths inversely proportional to their extinction coefficients.

Tissue engineering has provided an alternative strategy for the regeneration of functional tissues for drug screening and disease intervention. The central challenge in the development of mature and functional excitable tissues is to design and construct advanced conductive biomaterials that can guide cells to form electrically interconnected networks. The objective of this study was to develop reduced graphene oxide modified silk nanofibrous biomaterials with controllable surface deposition on the nanoscale. A vacuum filtration system was applied to attain reduced graphene oxide nanolayer deposition. The results demonstrate that with this method, a uniform and compact reduced graphene oxide nanolayer was formed, and the conductivity and nanofibrous morphology of the materials was well controlled. The composite nanofibrous scaffolds were applied for the engineering of cardiac tissues and demonstrated a great ability to promote tissue formation and functions, including the expression of cardiac-specific proteins, the formation of sarcomeric structures and gap junctions, and tissue contraction. External electrical stimulation further enhanced the maturation level of cardiac tissues cultured on these conductive scaffolds. All these results demonstrated the great potential of reduced graphene oxide functionalized silk biomaterials fabricated using our method for recapitulating electrical microenvironments for the regeneration of functional excitable tissues.