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The whitebacked planthopper (WBPH), Sogatella furcifera, and brown planthopper (BPH) Nilaparvata lugens Stå l are important sucking insects of rice (Oryza sativa L.) crops throughout the world. Rice 'B5', which has derived its resistance genes from the wild rice O. officinalis Wall ex Watt, is a line that is highly resistant to both WBPH and BPH. Previously, two resistance genes against BPH, Qbp1, and Qbp2 in 'B5' had been mapped onto chromosome 3 and chromosome 4, respectively. In this study, we employed a mapping population composed of 187 recombinant inbred lines (RILs), produced from a cross between 'B5' and susceptible variety 'Minghui63', to locate the WBPH and BPH resistance genes. A RFLP survey of the bulked extremes from the RIL population identified two genomic regions, one on chromosome 3 and the other on chromosome 4, likely containing the resistance genes to planthoppers. QTL analysis of the RILs further confirmed that two WBPH resistance genes were mapped on the same loci as Qbp1 and Qbp2, using a linkage map with 242 molecular markers distributed on 12 rice chromosomes. Of the two WBPH resistance genes, one designated Wbph7(t) was located within a 1.1-cM region between R1925 and G1318 on chromosome 3, the other designated Wbph8(t) was within a 0.3-cM region flanked by R288 and S11182 on chromosome 4. A two-way analysis of variance showed that two loci acted independently with each other in determining WBPH resistance. The results have significant implications in studying the interactions between sucking insects and plants and in breeding programs of resistance to rice planthoppers.

The 3 major subphenotypes observed in patients with nonsyndromic orofacial clefts (NSOFCs) are nonsyndromic cleft lip only (NSCLO), nonsyndromic cleft lip with palate (NSCLP), and nonsyndromic cleft palate only (NSCPO). However, the genetic architecture underlying NSCPO is largely unknown. Here we performed a 2-stage genome-wide association study (GWAS) on NSCPO and replication analyses of selected variants in other NSOFCs from the Chinese Han population. We identified a novel locus (15q24.3) and a known locus (1q32.2) where variants in or near the gene reached genome-wide significance (2.80 × 10−13 < P < 1.72 × 10−08) in a test for association with NSCPO in a case-control design. Although a variant from 15q24.3 was found to be significantly associated with both NSCPO and NSCLP, the direction of estimated effects on risk were opposite. Our functional annotation of the risk alleles within 15q24.3 coupled with previously established roles of the candidate genes within identified risk loci in periderm development, embryonic patterning, and/or regulation of cellular processes supports their involvement in palate development and the pathogenesis of cleft palate. Our study advances the understanding of the genetic basis of NSOFCs and provides novel insights into the pathogenesis of NSCPO.

We report a novel three-step strategy toward polyCTA for the synthesis of cylindrical polymer brushes via “CTA-shuttled” RAFT R-approach polymerization. Post functionalizations on the CTA residue are also discussed.

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Rapid urbanization is responsible for the increased vulnerability of land systems and the loss of many crucial ecosystem services. Land systems are typical complex systems comprised of different land use types which interact with each other and respond to external environment processes (such as urbanization), resulting in dynamics in land systems. This work develops a methodology approach by integrating complex networks and disruptive scenarios and applies it to a case study area (Wuhan City in China) to explore the effects of urbanization on land system structural vulnerability. The land system network topologies of Wuhan City during five time periods from 1990 to 2015 are extracted. Our results reveal that (1) the urban land expands at a higher speed than the urban population in Wuhan City; (2) the period of 2005–2010 has witnessed more land area conversions from ecological lands to urban land than other periods; (3) the land system is more vulnerable to intentional attacks on nodes with higher integrated node centrality and larger land area, such as paddy, dryland, and lake; and (4) the network efficiency of the land system would decline sharply if the area shrinkage of paddy, dryland, and lake is larger than 30%, 50%, and 20%, respectively. The results provide some insights into building a resilient urban land system, such as increasing the efficiency of existing urban land and controlling the shrinkage rate of important land use types. This study contributes to existing literature on complex networks by expanding its application in land systems, which highlight the potential of complex networks to capture the complexity, dynamics, heterogeneity, and emergent phenomena in land systems.

Public transit plays a key role in shaping the transportation structure of large and fast growing cities. To cope with high population and employment density, such cities usually resort to multi-modal transit services, such as rail, BRT and bus. These modes are strategically connected to form an effective transit network. Among the transit modes, bus stops need to be properly deployed to maintain an acceptable walking accessibility. This paper presents a hierarchical process for optimizing bus stop locations in the context of fast growing multi-modal transit services. Three types of bus stops are identified hierarchically, which includes connection stops, key stops and ordinary stops. Connection stops are generated manually to connect with other transit facilities. Key stops and ordinary stops are optimized with coverage models that are respectively weighted by network centrality measure and potential demand. A case study in a Chinese city suggests the hierarchical approach may generate more effective stop distribution.