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The material point method (MPM) enhanced with B-spline basis functions, referred to as B-spline MPM (BSMPM), is developed and demonstrated using representative quasi-static and dynamic example problems. Smooth B-spline basis functions could significantly reduce the cell-crossing error as known for the original MPM. A Gauss quadrature scheme is designed and shown to be able to diminish the quadrature error in the BSMPM analysis of largedeformation problems for the improved accuracy and convergence, especially with the quadratic B-splines. Moreover, the increase in the order of the B-spline basis function is also found to be an effective way to reduce the quadrature error and to improve accuracy and convergence. For plate impact examples, it is demonstrated that the BSMPM outperforms the generalized interpolation material point (GIMP) and convected particle domain interpolation (CPDI) methods in term of the accuracy of representing stress waves. Thus, the BSMPM could become a promising alternative to the MPM, GIMP, and CPDI in solving certain types of transient problems. KEYWORDS B-spline basis functions, Gauss quadrature, large deformation, material point method, mesh refinement, transient problems

Acute lung injury (ALI) and its more severe form, acute respiratory distress syndrome (ARDS), are life-threatening diseases that are associated with high mortality rates due to treatment limitations. Neutrophils play key roles in the pathogenesis of ALI/ARDS by promoting the inflammation and injury of the alveolar microenvironment. To date, in vivo functional approaches have been limited by the inaccessibility to the alveolar sacs, which are located at the anatomical terminal of the respiratory duct in mammals. We are the first to characterize the swim bladder of the zebrafish larva, which is similar to the mammalian lung, as a real-time in vivo model for examining pulmonary neutrophil infiltration during ALI. We observed that the delivery of exogenous materials, including lipopolysaccharide (LPS), Poly IC and silica nanoparticles, by microinjection triggered significant time- and dose-dependent neutrophil recruitment into the swim bladder. Neutrophils infiltrated the LPS-injected swim bladder through the blood capillaries around the pneumatic duct or a site near the pronephric duct. An increase in the post-LPS inflammatory cytokine mRNA levels coincided with the in vivo neutrophil aggregation in the swim bladder. Microscopic examinations of the LPS-injected swim bladders further revealed in situ injuries, including epithelial distortion, endoplasmic reticulum swelling and mitochondrial injuries. Inhibitor screening assays with this model showed a reduction in neutrophil migration into the LPS-injected swim bladder in response to Shp2 inhibition. Moreover, the pharmacological suppression and targeted disruption of Shp2 in myeloid cells alleviated pulmonary inflammation in the LPS-induced ALI mouse model. Additionally, we used this model to assess pneumonia-induced neutrophil recruitment by microinjecting bronchoalveolar lavage fluid from patients into swim bladders; this injection enhanced neutrophil aggregation relative to the control. In conclusion, our findings highlight the swim bladder as a promising and powerful model for mechanistic and drug screening studies of alveolar injuries.

ABSTRACT:The paper presents some experiments carried out as part of the virtual reconstruction of buildings just documented by partial sketches, or partially built, or no more existing, with the aim (a) to emphasize the use of a semantic construction of the digital model, not only as a means to modeling a building but as a cognitive system, (b) to show conceptual similarity between the treaties and BIM, (c) to propose new and more robust solutions to the 3D modeling from 2D drawings for CH artifacts, able to allow the verification of the assumptions used during the reconstruction pipeline, (d) to make use of interactive technical reference, typically real-time photorealistic rendering, for the visualization of three-dimensional model and of variants snapshots, managed by an iconic for illustrating the method of comparison and guided reading of model's characters of the steps taken.

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The present study explored the feasibility of using hexagonal-phase NaYF4:Ce,Tb,Gd nanocrystals as bimodal probes for fluorescence and magnetic resonance (MR) imaging. Using a facile and user-friendly strategy, the NaYF4:Ce,Tb,Gd nanocrystals were synthesized with good water dispensability, high quantum yield (26%), and decent MRT1relaxivity (r1=2.87 mM−1 s−1). The NaYF4:Ce,Tb,Gd NCs conjugated by folic acid presented great efficiency in fluorescence imaging of C6 glioma cellsin vitro. Meanwhile, inin vivoMR experiments on rats, the NaYF4:Ce,Tb,Gd NCs also significantly increasedT1signal in the liver, spleen, and kidney even with a low probe dose. The proposed NaYF4:Ce,Tb,Gd nanoprobes hold promise for simultaneous bimodal fluorescence and MR bioimaging.