No abstract

Developing easy-to-use and miniaturized detectors is essential for in-field monitoring of environmentally hazardous substances, such as the cyanotoxins. We demonstrated a differential fluorescent sensor array made of aptamers and single-stranded DNA (ssDNA) dyes for multiplexed detection and discrimination of four common cyanotoxins with an ordinary smartphone within 5 min of reaction. The assay reagents were preloaded and dried in a microfluidic chip with a long shelf life over 60 days. Upon the addition of analyte solutions, competitive binding of cyanotoxin to the specific aptamer-dye conjugate occurred. A zone-specific and concentration-dependent reduction in the green fluorescence was observed as a result of the aptamer conformation change. The aptasensors are fully optimized by quantification of their dissociation constants, tuning the stoichiometric ratios of reaction mixtures, and implementation of an internal intensity correction step. The fluorescent sensor array allowed for accurate identification and measurement of four important cyanotoxins, including anatoxin-a (ATX), cylindrospermopsin (CYN), nodularin (NOD), and microcystin-LR (MC-LR), in parallel, with the limit of detection (LOD) down to a few nanomolar (<3 nM), which is close to the World Health Organization's guideline for the maximum concentration allowed in drinking water. The smartphone-based sensor platform also showed remarkable chemical specificity against potential interfering agents in water. The performance of the system was tested and validated with real lake water samples that were contaminated with trace levels of individual cyanotoxins as well as binary, ternary, and quaternary mixtures. Finally, a smartphone app interface has been developed for rapid on-site data processing and result display.

Ac olorimetric sensor arrayh as been designed for the identification of and discrimination among aldehydes and ketones in vapor phase.D ue to rapid chemical reactions between the solid-state sensor elements and gaseous analytes, distinct color difference patterns were produced and digitally imaged for chemometric analysis.T he sensor arrayw as developed from classical spot tests using aniline and phenylhydrazine dyes that enable molecular recognition of aw ide variety of aliphatic or aromatic aldehydes and ketones,a s demonstrated by hierarchicalc luster,p rincipal component, and support vector machine analyses.T he aldehyde/ketonespecific sensors were further employed for differentiation among and identification of ten liquor samples (whiskies, brandy,v odka) and ethanol controls,s howing its potential applications in the beverage industry.

No abstract

Forensic detection of non-volatile nitro explosives poses a difficult analytical challenge. A colorimetric sensor comprising of ultrasonically prepared silica-dye microspheres was developed for the sensitive gas detection of cyclohexanone, a volatile marker of explosives 1,3,5-trinitro-1,3,5-triazinane (RDX) and 1,3,5,7-tetranitro-1,3,5,7-tetrazocane (HMX). The silica-dye composites were synthesized from the hydrolysis of ultrasonically sprayed organosiloxanes under mild heating conditions (150 • C), which yielded microspherical, nanoporous structures with high surface area (~300 m 2 /g) for gas exposure. The sensor inks were deposited on cellulose paper and given sensitive colorimetric responses to trace the amount of cyclohexanone vapors even at sub-ppm levels, with a detection limit down to~150 ppb. The sensor showed high chemical specificity towards cyclohexanone against humidity and other classes of common solvents, including ethanol, acetonitrile, ether, ethyl acetate, and ammonia. Paper-based colorimetric sensors with hierarchical nanostructures could represent an alternative sensing material for practical applications in the detection of explosives.