A novel polymycovirus isolating from plant-pathogenic fungus Colletotrichum gloeosporioides was identified. The virus were composed nine RNA segments, ranging from 699 bp to 2,444 bp, and except that RNA5 contains two open reading frames (ORF5-1 and ORF5-2), other RNA segement contain only one ORF. In addition,the proteins encoded by ORFs 1-8 were homologous to the protein encoded by ORFs 1-8 in Colletotrichum camelliae filamentous virus 1(CcFV-1) by BLASTp. The amino acid sequence between the RNA-dependent RNA polymerase (RdRp) encoded by ORF1 and the viral methyltransferase encoded by ORF3 shared 87.6% and 83.3% identity with CcFV-1. The proline-alanine-serine-rich protein (PASrp) encoded by ORF4 shows 86.6% sequence homology to that of CcFV-1. The sequence homology of proteins encoded by ORFs2, 5-1, 6, 7 and 8 with the corresponding proteins of CcFV-1 was 86.6%, 82.5%, 89.0%, 45.7% and 95.5%, respectively. However, RNA9 is a defective RNA of RNA2, missing the 1556 bp (nt 519 to nt 2074) intermediate fragment of RNA2. Phylogenetic analysis based on RdRp protein indicated that the virus, which is the phylogenetically closest relative of CcFV-1, clustered with members of the family Polymycoviridae, so we tentatively named it "Colletotrichum gloeosporioides polymycovirus virus 1" (CgPmV1). To our knowledge, this is the first report of a defective polymycovirus in C. gloeosporioides.
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