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Graphite oxide was prepared via the modified Hummers' method and graphene via chemical reduction. Deoxygenation efficiency of graphene oxide was compared among single reductants including sodium borohydride, hydrohalic acids, hydrazine hydrate, and vitamin C. Two-step reduction of graphene oxide was primarily studied. The reduced graphene oxide was characterized by XRD, TG, SEM, XPS, and Raman spectroscopy. Square resistance was measured as well. Results showed that films with single-step N 2 H 4 reduction have the best transmittance and electrical conductivity with square resistance of~5746 Ω/sq at 70% transmittance. This provided an experimental basis of using graphene for electronic device applications.