Background: Different mulches have variable effects on soil physicochemical characteristics, bacterial and fungal communities and ecosystem functions. However, the information about soil microbial diversity, community structure and ecosystem function in tea plantation under different mulching patterns was limited. In this study, we investigated bacterial and fungal communities of tea plantation soils under polyethylene film and peanut hull mulching using high-throughput 16S rRNA and ITS rDNA gene Illumina sequencing. Results: The results showed that the dominant bacterial phyla were Proteobacteria, Actinobacteria, Acidobacteria and Chloroflexi, and the dominant fungal phyla were Ascomycota, Mortierellomycota and Basidiomycota in all samples, but different mulching patterns affected the distribution of microbial communities. At the phylum level, the relative abundance of Nitrospirae in peanut hull mulching soils (3.24%) was significantly higher than that in polyethylene film mulching soils (1.21%) in bacterial communities, and the relative abundances of Mortierellomycota and Basidiomycota in peanut hull mulching soils (33.72, 21.93%) was significantly higher than that in polyethylene film mulching soils (14.88, 6.53%) in fungal communities. Peanut hull mulching increased the diversity of fungal communities in 0-20 cm soils and the diversity of bacterial communities in 20-40 cm soils. At the microbial functional level, there was an enrichment of bacterial functional features, including amino acid transport and metabolism and energy production and conversion, and there was an enrichment of fungal functional features, including undefined saprotrophs, plant pathogens and soils aprotrophs. Conclusions: Unique distributions of bacterial and fungal communities were observed in soils under organic mulching. Thus, we believe that the organic mulching has a positive regulatory effect on the soil bacterial and fungal communities and ecosystem functions, and so, is more suitable for tea plantation.
Autonomous vehicles (AV) can not only improve traffic safety and congestion, but also have strategic significance for the development of the transportation industry. With the continuous updating of core technologies such as artificial intelligence, sensor detection, synchronous positioning, and high‐precision mapping, the development of AV has been promoted. When 5G network is combined with Internet of Vehicles, the problems of AV can be solved by taking advantage of 5G ultra‐large bandwidth, low latency and high reliability. However, when the user controls the vehicle remotely, a real‐time and reliable authentication process is needed, while minimizing the overhead of security protocols. Therefore, this article proposes a practical and secure multifactor user authentication protocol for AV in 5G network. By introducing non‐interactive zero‐knowledge proof technology and physical uncloning function, the protocol completes mutual authentication and key agreement without revealing any sensitive information. The article proves the security of the protocol through BAN logic and the simulation of Scyther. And it can resist malicious attacks and provide more security features. The informal security analysis shows that the protocol can meet the proposed security requirements. Finally, we evaluate the efficiency of the protocol, and the results show that the protocol can provide better performance.
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