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Nowadays, biocatalysts have received much more attention in chemistry regarding their potential to enable high efficiency, high yield, and eco-friendly processes for a myriad of applications. Nature’s vast repository of catalysts has inspired synthetic chemists. Furthermore, the revolutionary technologies in bioengineering have provided the fast discovery and evolution of enzymes that empower chemical synthesis. This article attempts to deliver a comprehensive overview of the last two decades of investigation into enzymatic reactions and highlights the effective performance progress of bio-enzymes exploited in organic synthesis. Based on the types of enzymatic reactions and enzyme commission (E.C.) numbers, the enzymes discussed in the article are classified into oxidoreductases, transferases, hydrolases, and lyases. These applications should provide us with some insight into enzyme design strategies and molecular mechanisms.

BACKGROUND: To contribute towards understanding the relationship of structure and bioactivity, a proteinbound acidic polysaccharide named TPC3-1 was isolated and purified from low-grade green tea (Camellia sinensis L.). The homogeneity and weight average molecular weight of TPC3-1 was determined by agarose gel electrophoresis and high-performance gel permeation chromatography. The monosaccharide and amino acid composition of TPC3-1 were analysed by gas chromatography and an amino acid analyser. The molecular structure of TPC3-1 was characterised by Fourier transform infrared spectroscopy, 13 C nuclear magnetic resonance spectroscopy and atomic force microscopy.

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