MicroRNAs (miRNAs) are a group of non-protein‑coding, short single-stranded RNAs, which are considered as promising molecular markers and therapeutic targets in several cancers. The present study explored the expression patterns and functional roles of miR‑202 in non‑small cell lung cancer (NSCLC). The expression levels of miR‑202 were determined in NSCLC tissues and cell lines using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The functional impact of miR‑202 overexpression on NSCLC cell viability, migration and invasion were evaluated using Cell Counting Kit‑8 reagent and Transwell migration and invasion assays, respectively. The molecular mechanism underlying the tumor suppressive roles of miR‑202 on NSCLC was examined using bioinformatics analysis, luciferase reporter assay, RT‑qPCR and western blot analysis. In addition, signal transducer and activator of transcription (STAT) 3 was overexpressed to investigate the impact on miR‑202‑mediated tumor suppression in NSCLC. The results indicated that miR‑202 was downregulated in NSCLC tissues and cell lines, and was associated with tumor node metastasis stage and lymph node metastasis. Exogenous miR‑202 expression reduced NSCLC cell viability, migration and invasion. Furthermore, STAT3 was identified as a direct target gene of miR‑202 in NSCLC. STAT3 overexpression improved miR‑202‑impaired cell viability, migration and invasion. In conclusion, the present study revealed novel anticancer effects induced by miR‑202 upregulation in NSCLC, and indicated that STAT3 may be a molecular target of miR‑202.

Osteoarthritis (OA) is a common and troublesome disease among the elderly, and is characterized by extracellular matrix (ECM) degradation. The function of the long non-coding RNA X-inactive-specific transcript (XIST) and its working mechanism in ECM degradation remains unclear. In the present study, XIST was revealed to be upregulated in OA specimens and in articular chondrocytes (ACs) derived from OA tissue (AC/OA) and interleukin-1β (IL-1β)-treated ACs. Loss-of-function experiments demonstrated that downregulation of XIST suppressed the degradation of the ECM in AC/OA and AC/IL-1β-5.0 cells. Furthermore, XIST, matrix metalloproteinase 13 (MMP-13) and ADAM metallopeptidase with thrombospondin type 1 motif 5 (ADAMTS5) were identified as targets of microRNA (miR)-1277-5p, and the reciprocal inhibitive effect between XIST and miR-1277-5p was elucidated. Furthermore, the role of XIST in ECM degradation was confirmed to be functioning as a competing endogenous RNA (ceRNA) of miR-1277-5p. Finally, the protective effect of the downregulation of XIST on ECM degradation was verified in an OA rat model. In conclusion, the present study suggests that XIST promotes MMP-13 and ADAMTS5 expression, indicating ECM degradation, by functioning as a ceRNA of miR-1277-5p in OA. The present study proposed a novel potential target with a new working mechanism in molecular treating of OA.

Objective: To study the effects of single-nucleotide polymorphisms of the OPRM1 and ABCB1 genes on the analgesic effect and consumption of sufentanil after thoracoscopic-assisted radical resection of lung cancer.Methods: A total of 225 Chinese Han nationality patients undergoing thoracoscopic-assisted radical resection of lung cancer were enrolled in the present study. Among them, 132 were males (58.67%) and 93 (41.33%) were females having American Society of Anesthesiologists statuses classified as grades I or II. The rs1799971, rs563649 and rs1323040 genotypes of the OPRM1 gene and rs2032582, rs1045642 and rs1128503 genotypes of the ABCB1 gene were detected by Sanger sequencing. The state anxiety index and pressure pain threshold were assessed preoperatively. Sufentanil was administered intravenously to maintain anesthesia. The doses and side effects of sufentanil consumed 6 h (T1), 24 h (T2) and 48 h (T3) after surgery were recorded.Results: The sufentanil doses at T1, T2 and T3 were significantly higher in radical-operation lung cancer patients with mutant homozygous rs1799971 and rs1323040 loci in the OPRM1 gene and rs2032582 and rs1128503 loci in the ABCB1 gene. The doses of sufentanil consumed by mutant heterozygous lung cancer patients at T1, T2 and T3 were significantly higher than those consumed by patients without mutations, and the differences were statistically significant (P<0.05). There was no significant difference in sufentanil doses consumed by lung cancer patients with mutant homozygous, mutant heterozygous and wild-type rs563649 locus of the OPRM1 gene and rs1045642 locus of the ABCB1 gene at T1, T2 and T3 (P>0.05). There was no significant difference in the visual analog scale scores at T1, T2 and T3 for different genotypes of OPRM1 and ABCB1 genes in lung cancer patients (P>0.05). No significant difference was found between the adverse reactions of OPRM1 and ABCB1 genotypes in patients undergoing radical resection of lung cancer (P>0.05).Conclusion: The rs1799971 and rs1323040 polymorphisms of the OPRM1 gene and rs2032582 and rs1128503 polymorphisms of the ABCB1 gene are related to the analgesic effect and consumed dose of sufentanil in Chinese Han patients undergoing radical operation of lung cancer.

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In magnetic confinement fusion (MCF), the plasma always exhibits an anisotropic temperature distribution, which may impact not only the plasma dynamics but also the nuclear reaction process. Here, through theoretical derivations and self-consistent particle-in-cell simulations with the newly-developed nuclear reaction and alpha particle energy deposition calculation modules, we find that, if considering the plasma has an anisotropic temperature distribution, the fusion energy gain factor ($Q$) of MCF is significantly modified, where both the deuteron-triton nuclear reactivity and the alpha particle energy deposition fraction are heavily influenced. The simulation results show that, under the International Thermonuclear Experimental Reactor (ITER) condition, if the plasma temperature anisotropy ratio can reach 0.1, i.e., the plasma perpendicular temperature component is one-tenth of its parallel component corresponding to the ambient magnetic field direction, the $Q$-value of ITER can be increased from the originally-designed 5 to about 10, with doubled enhancement.

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The newly discovered Yongxin Au deposit in the Lesser Xing'an Range of north‐eastern Heilongjiang Province, NE China, has a resource of ~20 t with a grade of 4.1 g/t Au. The deposit is hosted by Carboniferous syenogranite and mylonite, and Cretaceous diorite porphyry and granite porphyry. The syenogranite has a LA‐ICP‐MS 206Pb/238U zircon age of 316 ± 2 Ma, and both the granite porphyry and diorite porphyry yield an age of 119 ± 1 Ma. The Rb–Sr isochron age of Au‐bearing pyrite from hydrothermal breccia‐type ore is 107 ± 4 Ma, and this is interpreted as the mineralization age of the Yongxin deposit. In combination with published geochronological data, we argue that epithermal Au mineralization in NE China formed during the period ca. 124 to 107 Ma and was produced during rollback of Palaeo‐Pacific oceanic slab. The Cretaceous epithermal Au deposits are distributed in the Erguna Massif, Lesser Xing'an Range, Zhangguangcai Range, and Jiamusi and Nadanhada terranes. In combination with the coeval porphyry Mo deposits present in these areas, we propose that the Erguna Massif, Lesser Xing'an Range, north‐eastern Great Xing'an Range, and eastern Jiamusi and Nadanhada terranes, where only epithermal gold deposits were discovered up to date, might be prospective for concealed porphyry Cu or Mo deposits.