Developing rapid and diverse microbial mutation tool is of importance to strain modification. In this review, a new mutagenesis method for microbial mutation breeding using the radio-frequency atmospheric-pressure glow discharge (RF APGD) plasma jets is summarized. Based on the experimental study, the helium RF APGD plasma jet has been found to be able to change the DNA sequences significantly, indicating that the RF APGD plasma jet would be a powerful tool for the microbial mutagenesis with its outstanding features, such as the low and controllable gas temperatures, abundant chemically reactive species, rapid mutation, high operation flexibility, etc. Then, with the RF APGD plasma generator as the core component, a mutation machine named as atmospheric and room temperature plasma (ARTP) mutation system has been developed and successfully employed for the mutation breeding of more than 40 kinds of microorganisms including bacteria, fungi, and microalgae. Finally, the prospect of the ARTP mutagenesis is discussed.
Power oscillation and current quality are the important performance targets for the grid-connected inverter under unbalanced grid faults. Firstly, the inherent reason for the current harmonic and power oscillation of the inverter is discussed with a quantitative analysis. Secondly, a new control strategy is proposed to achieve the coordinate control of power and current quality without the need for a phase-locked loop (PLL) or voltage/current positive/negative sequence extraction calculation. Finally, the experimental tests are conducted under unbalanced grid faults, and the results verify the effectiveness of the propose method.
This paper presents a new control strategy of three-phase grid-connected inverter for the positive sequence voltage recovery and negative sequence voltage reduction under asymmetrical grid faults.Unlike the conventional control strategy based on an assumption that the network impedance is mainly inductive, the proposed control strategy is more flexible and effective by considering the network impedance impact, which is of great importance for the high penetration of grid-connected renewable energy systems into low-voltage grids. The experimental tests are carried out to validate the effectiveness of the proposed solution for the flexible voltage support in a low voltage grid, where the network impedance is mainly resistive.
Consequences of submarine landslides include both their direct impact on offshore infrastructure, such as subsea electric cables and gas/oil pipelines, and their indirect impact via the generated tsunami. The simulation of submarine landslides and their consequences has been a long-standing challenge majorly due to the strong coupling among sliding sediments, seawater and infrastructure as well as the induced extreme material deformation during the complete process. In this paper, we propose a unified finite element formulation for solid and fluid dynamics based on a generalised Hellinger-Reissner variational principle so that the coupling of fluid and solid to be achieved naturally in a monolithic fashion. In order to tackle extreme deformation problems, the resulting formulation is implemented within the framework of the particle finite element method. The correctness and robustness of the proposed unified formulation for single-phase problems (e.g. fluid dynamics problems involving Newtonian/Non-Newtonian flows and solid dynamics problems) as well as for multi-phase problems (e.g. two-phase flows) are verified against benchmarks. Comparisons are carried out against numerical and analytical solutions or experimental data that are available in literatures. Last but not least, the possibility of the proposed approach for modelling submarine landslides and their consequences is demonstrated via a numerical experiment of an underwater slope stability problem. It is shown that the failure and postfailure process of the underwater slope can be predicted in a single simulation with its direct 2 threat to a nearby pipeline and indirect threat by generating tsunami being estimated as well.
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