No abstract

Objective: This study aims to identify the ideal saliva collection technique and propose a newly sensitive approach to achieve daily non-invasive blood glucose monitoring for healthy people. Method: A total amount of six methods were employed for collecting saliva glucose in 20 healthy participants in the morning (9:00), before lunch (11:00), after lunch (14:00) and in the evening (17:00). Ultra-micro ultraviolet spectrophotometer was used to launch a new saliva glucose detection system in measuring the saliva glucose concentration.Results: The newly developed glucose detection system showed a statistically significant linear relationship in the range of 0.1-6 mg/dl with R2 = 0.999. Besides, the parotid saliva glucose concentration was higher than both the sublingual/submandibular saliva and the whole saliva. The parotid glucose concentration fluctuating after meal, and the sublingual/submandibular glucose concentration steady. Conclusions: The detection limit was 1/110 of the average blood glucose concentration of healthy people when fasting, which fully met the sensitivity requirements for detecting the saliva glucose concentration of healthy people. Besides, the collection method was an important factor that affected the saliva glucose concentration. The unstimulated parotid saliva glucose may be easier to reflect blood glucose concentration, which provided a reference for the evaluation of the health status of subjects and the early prediction of diabetes mellitus.