Background Cesarean scar pregnancy (CSP) is life-threatening, and the number of patients with CSP is rapidly increasing in China. However, the pathogenesis of CSP is still unclear due to the lack of macroscopic data due to spatial and temporal constraints. Therefore, the aim of this study was to explore the key regulatory molecules and mechanisms of CSP through a multi-omics approach.MethodsProteomics was used to detect proteins expression in deciduas and villus from six clinical patients clinical patients. Gene expression datasets were downloaded using the GEO and SRA databases. Bioinformatics analysis was performed in a series of databases and software such as DAVID, Metascape and STRING. Data analysis was performed using SPSS 27, and P < 0.05 was statistically significant.ResultsThe occurrence of CSP has common DEGs with cesarean delivery (CD) and embryo implantation (EI). Enrichment analysis revealed that Biological Process and the KEGG pathway are associated with the adhesion process, with the involvement of ITGB3, ITGA2B and VTN. ITGB3 expression was significantly downregulated after CD compared to spontaneous delivery, and then increased after another pregnancy compared to normal pregnancy.ConclusionsThe occurrence of CSP is inevitably associated with CD and EI. ITGB3, ITGA2B, and VTN may act as key molecules for CSP by activating the focal adhesion signaling pathway. The rebound effect of ITGB3 is a key regulatory mechanism for CSP formation.
Dolphins are marine mammals with unique anatomical structures in their airways. These particular respiratory structures make dolphins very sensitive to microorganisms in the air, and the habitats of dolphins in captivity and in the wild are quite different. This causes captive dolphins to suffer from illnesses, especially respiratory diseases. Previous studies have shown that the respiratory flora plays an essential role in the health of humans and animals. Therefore, by comparative analysis of the respiratory flora of wild dolphins and dolphins in captivity, we want to find the flora related to dolphins’ respiratory diseases, and try to improve dolphin health through flora homeostasis. For this purpose, we performed 16S rRNA gene sequencing and analyses. We found that Proteobacteria, Firmicutes, Actinobacteria, Campilobacterota, Paracoccus, and other flora differed between wild and healthy and sub-healthy captive dolphins. Some of them are the dominant flora for promoting health, and the others may be essential pathogenic bacteria in the sub-healthy state of dolphins in captivity.
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