Purpose Exercise training and some herbal components have an anticancer function and can suppress tumor growth. However, the role of these protective factors in altering breast cancer–related gene expression is still unknown. Thus, this study aimed to assess the effect of 4 wk of high-intensity interval training (HIIT) and saffron (Crocus sativus L.) aqueous extract (SAE) on Sirtuin-1 (SIRT1), human telomerase reverse transcriptase (hTERT), and p53 gene expression in female mice breast tumor tissue induced by 4T1 cell line. Methods This study was performed on female BALB/c mice. The 4T1 breast cancer cells were subcutaneously implanted, and mice were randomly sorted into the following groups: control, HIIT, SAE, HIIT + SAE (n = 10 mice per group), and sham (n = 4 mice per group). Mice were sacrificed at the end of the intervention period, and the expression of SIRT-1, hTERT, and p53 was determined by real-time polymerase chain reaction. Results The mRNA level of SIRT1 was increased in the HIIT + SAE group compared with the HIIT and control groups (P = 0.007 and P = 0.03, respectively). Moreover, the amount of mRNA of p53 was increased after a 4-wk HIIT compared with the control and HIIT + SAE groups in tumor tissue (P = 0.03 and P = 0.02, respectively). No change was found in the mRNA expression of hTERT between groups (P = 0.92). Conclusions These findings suggest that HIIT may reduce tumor burden through the upregulation of p53 associated with tumor suppression pathway. In contrast, the combination of HIIT and SAE did not alter p53 and SIRT1 expression levels and may suppress tumor growth by other mechanisms.
SummaryStudy aim: The purpose of this study was to compare intake of antioxidant vitamins (A, C, E) in young women with various levels of physical activity.Material and methods: The study included 3 groups of females: 40 women with no extra physical activity (Sedentary), 40 women involved in regular, moderate sports activities (Recreationally Active) and 40 female athletes competing at an international level (Athletes). Participants’ diet was assessed on the basis of 3-day diet records which were analysed using the computer program ‘Dieta 5.0’. Body composition was evaluated by the bioelectrical impedance analysis (BIA) method.Results: Athletes were characterized by the lowest body fat and highest lean body mass in comparison with the other groups. They also showed the highest intake of energy, proteins and carbohydrates compared to Active and Sedentary women. The consumption of vitamin A amounted to 180.0% of RDA in Athletes, 98.8% in Recreationally Active and 97.8% in Sedentary women. Vitamin C intake in Athletes equalled 275.0% of RDA, whereas the deficiency of this vitamin was observed in Recreationally Active and Sedentary women (62.3% and 46.1% of RDA, respectively). The study groups showed consumption of vitamin E at the level of 146.7% of AI in Athletes, 115.0% in Recreationally Active and 111.3% in Sedentary women.Conclusion: Athletes consumed excessive amounts of antioxidant vitamins. Recreationally Active and Sedentary women met the demand for vitamin A and E, but the intake of vitamin C was not sufficient, which could lead to health problems. The current findings also indicate that energy intake was at a low level regardless of the study group.
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