In
this study, a biosensor assembly based on microelectrode arrays
(MEAs) modified with PtNPt/MWCNT-PEDOT:PSS nanocomposites is presented
to synchronously detect the dopamine (DA) and electrophysiological
activities in rat brains. Different morphological and electrochemical
characterizations were conducted to show the excellent mechanical
and electrical properties of the as-prepared probes. The developed
biosensors realized the sensitive and selective detection of DA with
the existence of significant interferences such as uric acid (UA),
ascorbic acid (AA), glutamate (Glu), and 3,4-dihydroxyphenylacetic
acid (DOPAC). Calibration curve for the DA response was linear with
the concentration from 0.05 μM to 79 μM (R = 0.999), with a sensitivity of 30.561 pA/μM and detection
limit as low as 50 nM. Finally, the proposed microelectrode was applied
to be implanted into the cortex and caudate putamen (CPU) of rats,
which was demonstrated to stably measure the synchronous neurochemical
and neurophysiological changes caused by 72 h sleep deprivation. The
in vivo measuring results showed that the sleep deprivation increased
the DA release and neural spike activity in both cortex and CPU. The
local field potential (LFP) power in the delta and theta band was
significantly increased as well. These changes in brain may reflect
the brain’s adaptive reaction toward the side effects induced
by sleep deprivation and may partially explain the mechanism of forced
wakefulness in the presence of accumulated sleep pressure.
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