This research was designed to elucidate the role of exopolysaccharides (EPS) producing bacterial strains for the amelioration of drought stress in wheat. Bacterial strains were isolated from a farmer’s field in the arid region of Pakistan. Out of 24 isolated stains, two bacterial strains, Bacillus subtilis (Accession No. MT742976) and Azospirillum brasilense (Accession No. MT742977) were selected, based on their ability to produce EPS and withstand drought stress. Both bacterial strains produced a good amount of EPS and osmolytes and exhibited drought tolerance individually, however, a combination of these strains produced higher amounts of EPS (sugar 6976 µg/g, 731.5 µg/g protein, and 1.1 mg/g uronic acid) and osmolytes (proline 4.4 µg/mg and sugar 79 µg/mg) and significantly changed the level of stress-induced phytohormones (61%, 49% and 30% decrease in Indole Acetic Acid (IAA), Gibberellic Acid (GA), and Cytokinin (CK)) respectively under stress, but an increase of 27.3% in Abscisic acid (ABA) concentration was observed. When inoculated, the combination of these strains improved seed germination, seedling vigor index, and promptness index by 18.2%, 23.7%, and 61.5% respectively under osmotic stress (20% polyethylene glycol, PEG6000). They also promoted plant growth in a pot experiment with an increase of 42.9%, 29.8%, and 33.7% in shoot length, root length, and leaf area, respectively. Physiological attributes of plants were also improved by bacterial inoculation showing an increase of 39.8%, 61.5%, and 45% in chlorophyll a, chlorophyll b, and carotenoid content respectively, as compared to control. Inoculations of bacterial strains also increased the production of osmolytes such asproline, amino acid, sugar, and protein by 30%, 23%, 68%, and 21.7% respectively. Co-inoculation of these strains enhanced the production of antioxidant enzymes such as superoxide dismutase (SOD) by 35.1%, catalase (CAT) by 77.4%, and peroxidase (POD) by 40.7%. Findings of the present research demonstrated that EPS, osmolyte, stress hormones, and antioxidant enzyme-producing bacterial strains impart drought tolerance in wheat and improve its growth, morphological attributes, physiological parameters, osmolytes production, and increase antioxidant enzymes.

Tannery-affected surface soils from 72 sampling sites from industrial area of Sialkot district, Pakistan, were collected and analyzed for nine physicochemical parameters, nine heavy metals, and four macro-nutrients. Most of the soils were poor in organic matter (0.11-2.98 %), basic in nature with pH (7.1-10.6) and electrical conductivity (1.2-17.9 mS/ cm). Mean concentration of total dissolved solids, Cl 1-, alkalinity, NO 3 1 -N, salinity, and PO 4 3-was 3,093 mg/L, 6,587, 3,929, 301.3, 10.3, and 1.7 mg/kg. The results showed that concentration of macro-nutrients was in the order: Na[Mg[K[Ca whereas heavy metals followed the order: Cr [Fe[Ni[Mn[Cu[Zn[Co[Pb[Cd. Factor analysis based on principal component analysis, cluster analysis, and correlation analysis identified contribution of metals from tannery effluents, agrochemicals, automobiles exhaust, and natural weathering processes. Tannery-affected soils were enriched with Cd followed by Cr, Pb, Ni, Cu, Co, Zn, and Mn. Geo-accumulation index (I geo ) classified the soil samples in unpolluted to moderately polluted categories. Metal pollution index provided better estimation of heavy metal pollution as compared to pollution load index. Ecological risk index showed high potential ecological risk associated with Cd and Cr with mean concentrations above respective average shale/ background values. The results are useful for heavy metals source identification, enrichment, risk assessment, and management of tannery-affected soils and can contribute to monitoring programs at regional levels.

No abstract

No abstract

The effect of ultrasound pretreatment using Single Frequency Counter Current Ultrasound (SFCCU) on the enzymolysis of tea residue protein (TRP) extracted with sodium hydroxide was investigated. The concentration of TRP hydrolysate, enzymolysis kinetics and thermodynamic parameters after SFCCU pretreatment were determined and compared with traditional enzymolysis. The results indicated that both ultrasound assisted and traditional enzymolysis conformed to first-order kinetics within the limits of the studied parameters. Temperature and sonication had affirmative effect on the enzymolysis of TRP with temperature yielding greater impact. Michaelis constant ( ) in ultrasonic pretreated enzymolysis decreased by 32.7% over the traditional enzymolysis. The highest polypeptide concentration of 24.12 mg ml was obtained with the lowest energy requirement at improved conditions of 50 g L of TRP, alcalase concentration of 2000 U g, time of 10 min and temperature of 50 °C for the ultrasonic treated enzymolysis. The values of reaction rate constant () for TRP enzymolysis increased by 78, 40, 82 and 60% at 20, 30, 40 and 50 °C, respectively. The thermodynamic properties comprising activation energy (), change in enthalpy ) and entropy) were reduced by ultrasound pretreatment whereas Gibbs free energy ) was increased.

Purpose: To determine the effects of dates vinegar on blood biochemical and hematological parameters in type 2 diabetic subjects. Methods: Current research focused on fifty-five subjects having blood sugar more than126 mg/dL. Participants ingested dates vinegar (20 mL) daily into their normal diets for a period of 10 weeks. Glycated hemoglobin (HbA1c), fasting blood sugar (FBS), total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), creatinine (Cr), urea, complete blood count (CBC), alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), potassium and folate levels were analyzed before, after 5 weeks and after the experiment Results: Dates vinegar improved the blood concentrations of HbA1c (6.80 ±2.34 to 6.17 ± 2.14 (%)),

The study aims to determine drying of sweet potatoes using multifrequency ultrasound (US) pretreatments (20, 40, and 60 kHz) at three different infrared (IR) drying temperatures (60, 70, and 80°C) and evaluate the phytochemical and textural quality of the dried product. Drying time was significantly decreased in moderate US frequency (40 kHz) at 70°C with the increasing drying temperature. Comparing to the fresh samples, the dried samples showed the highest amount of phytochemical contents. The antioxidant activity of the samples increased especially at 60 kHz and 80°C, while US‐IR treatments shown a positive impact on total carotenoids contents and β‐carotene. For phenolic compounds, Ellagic acid and Rutin were quantified in higher amount while Quercetin‐3‐rhamnoside and Quercetin 3‐β‐D‐glucoside were two new compounds identified for the first time in sweet potatoes. FTIR spectra showed the successful synthesis of OH group and phenolics in samples treated with the US at 20 kHz. Practical applications This study investigated the effects of multifrequency ultrasound with different infrared drying temperatures. The study provides evidence that infrared drying application in synergy with ultrasonic pretreatments can improve drying efficiency and food quality much better than using each method alone. Total phenolic contents and total flavonoid contents remained stable at US 40 kHz and 60°C conditions. The findings showed that moderate ultrasound frequency (40 kHz) at 60°C improved phytochemical properties while antioxidant activities showed better preservation response at 80°C with 60 kHz. In addition, the samples treated with the same US treatment at 40 kHz showed less cell breakage in SEM analysis.

Phytoremediation is a promising cleanup technology for contaminated soils, groundwater, and wastewater that is both low-tech and low-cost. The objective of this study was to investigate the ameliorative effect of phytoremediation on municipal wastewater (MWW). For this purpose, a phytoremediation garden was established using different aquatic plants species [Pistia stratiotes, Eichhornia crassipess, Hydrocotyle umbellatta, Lemna minor, Tyhpa latifolia, and Scirpus acutus] in seven earthen pond systems (P1-P7) for the cyclic treatment of MWW. The physico-chemical analysis of MWW was carried out before and after the cyclic phytoremediation. Results showed that pH, EC and turbidity of MWW were reduced by 5.5%, 33.7%, and 93.1%, respectively after treatment (from P1 to P7). Treatment system also reduced total dissolved solids (TDS) by 35.2%, Cl by 61%, HCO3 by 29.2%, hardness by 45.7%, Ca by 32.3% and Mg by 55.9%. Nitrate concentration was reduced by 77.6% but SO4 was enhanced slightly. An ameliorative combined effect of wetland plants namely L. minor, T. latifolia, and S. acutus on MWW was noticed. Sequential phytoremediation with a mixture of plants was more effective than that relying only on a single plant species.