The ability of Trichoderma viride to synthesize extracellular enzymes (Lacase enzyme) using a substrate that contains media was examined. This isolate generated laccase at its greatest level (0.166U/ml) in liquid medium.  This study was  demonstrated the production, purification, and characterisation of the laccase enzyme from T.viride. The results showed that 96 hours was the optimum period of time for Laccase to incubate from this fungus. Laccase displayed the maximum level of activity at pH 4.5 and temperature 30 °C. The results revealed that the best ratio for laccase precipitation was 90%  by using ammonium sulphate.  In addition, for the purification of laccase enzyme, one peak of Laccase was appeared  in gel filtration purified from T.viride, while 2 peaks of Laccase were seen in ion exchange chromatography. According to the findings, the molecular weight of T. viride Laccase using SDS-PAGE was roughly 66KD under denaturation conditions.

Nanotechnology is quickly becoming one of the most essential and transformative areas of science. Physical, chemical, mechanical, and biological approaches are all used to create nanoparticles. Plants or microorganisms are frequently used in biological methods of metal ion reduction because they are clean, nontoxic, safe, biocompatible, and ecologically friendly. Fourir transform infra-red spectroscopy (FT–IR), scaning electron microscopy (SEM), and X-ray diffraction spectroscopy were described to analyze the nanoparticles generated (XRD). Nanoparticles (NPs) made from fungi have a diverse range of bio catalytic techniques, including enzyme immobilization for increased enzymatic activity. Silver (Ag) NPs made from fungi were discovered to have a benign effect in a wound and a thermal wound, and to have anti-mosquito, antibacterial, and antifungal properties

Although Aeromonas are common in aquatic habitats and have been marked as an arising risk to human health, some information dealing with antibiotic resistance profiles and virulence factor genes involved in pathogenicity are understood. The objective of this study was to evaluate the resistance profile for aquatic A. sobria and to identify the virulence factor genes. Aeromonas sobria isolates were collected from AL-Hillah River in Babel near the hospital swage water from January until May 2021. VITEK 2 system was used to diagnose isolates of the anaerobic G-ve A. Sobria bacteria, which were then confirmed by PCR for 16S RNA. Eight different groups of antibiotics were examined in A. sobria isolates using the disk diffusion method on a Mueller-Hinton agar. Genes encoding for virulence factor genes (act, ast, ela, alt, lip, asa, hly, and aer) were detected using conventional PCR. The isolates showed resistance to β-lactam drugs, while they were susceptible to ciprofloxacin, erythromycin, tetracycline, clindamycin and presented susceptibility to the gentamycin at rate 57%. Gel electrophoresis results of PCR products variably displayed clear bands for virulence factor genes (act, ast, ela, alt, lip, and asa), previously reported to be associated with some diseases. This is the first study in provinces of middle Iraqi dealing with aquatic A. sobria that evaluated the antibiotic sensitivity and investigated the virulence factor genes, including cytotoxic enterotoxins and enzymes. Virulence factor genes detection and 16S RNA gene for species identification were achieved by designing specific primers in the present study.