Highlights d A complex of STTs acts in sorting chloroplast Tat substrates to the thylakoids d Tat substrate activates the STT complex to undergo phase separation d Hcf106 reverses phase separation and facilitates cargo translocation

Generating natural and accurate descriptions in image captioning has always been a challenge. In this paper, we propose a novel recall mechanism to imitate the way human conduct captioning. There are three parts in our recall mechanism : recall unit, semantic guide (SG) and recalled-word slot (RWS). Recall unit is a text-retrieval module designed to retrieve recalled words for images. SG and RWS are designed for the best use of recalled words. SG branch can generate a recalled context, which can guide the process of generating caption. RWS branch is responsible for copying recalled words to the caption. Inspired by pointing mechanism in text summarization, we adopt a soft switch to balance the generated-word probabilities between SG and RWS. In the CIDEr optimization step, we also introduce an individual recalled-word reward (WR) to boost training. Our proposed methods (SG+RWS+WR) achieve BLEU-4 / CIDEr / SPICE scores of 36.6 / 116.9 / 21.3 with cross-entropy loss and 38.7 / 129.1 / 22.4 with CIDEr optimization on MSCOCO Karpathy test split, which surpass the results of other state-of-the-art methods.

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Fe–S clusters are ancient, ubiquitous and highly essential prosthetic groups for numerous fundamental processes of life. The biogenesis of Fe–S clusters is a multistep process including iron acquisition, sulfur mobilization, and cluster formation. Extensive studies have provided deep insights into the mechanism of the latter two assembly steps. However, the mechanism of iron utilization during chloroplast Fe–S cluster biogenesis is still unknown. Here we identified two Arabidopsis DnaJ proteins, DJA6 and DJA5, that can bind iron through their conserved cysteine residues and facilitate iron incorporation into Fe–S clusters by interactions with the SUF (sulfur utilization factor) apparatus through their J domain. Loss of these two proteins causes severe defects in the accumulation of chloroplast Fe–S proteins, a dysfunction of photosynthesis, and a significant intracellular iron overload. Evolutionary analyses revealed that DJA6 and DJA5 are highly conserved in photosynthetic organisms ranging from cyanobacteria to higher plants and share a strong evolutionary relationship with SUFE1, SUFC, and SUFD throughout the green lineage. Thus, our work uncovers a conserved mechanism of iron utilization for chloroplast Fe–S cluster biogenesis.

Adventitious roots form from non-root tissues as part of normal development or in response to stress or wounding. The root primordia form in the source tissue, and during emergence the adventitious roots penetrate the inner cell layers and the epidermis; however, the mechanisms underlying this emergence remain largely unexplored. Here, we report that a regulatory module composed of the AP2/ERF transcription factor ABSCISIC ACID INSENSITIVE 4 (ABI4), the MAP kinases MPK3 and MPK6, and the phosphatase PP2C12 plays an important role in the emergence of junction adventitious roots (J-ARs) from the root-hypocotyl junctions in Arabidopsis thaliana. ABI4 negatively regulates J-AR emergence, preventing the accumulation of reactive oxygen species and death of epidermal cells, which would otherwise facilitate J-AR emergence. Phosphorylation by MPK3/MPK6 activates ABI4 and dephosphorylation by PP2C12 inactivates ABI4. MPK3/MPK6 also directly phosphorylate and inactivate PP2C12 during J-AR emergence. We propose that this ''double-check'' mechanism increases the robustness of MAP kinase signaling and finely regulates the local programmed cell death required for J-AR emergence.

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