“
Candidatus
Parvarchaeales” microbes may represent a lineage uniquely distributed in extreme environments such as AMD and hot springs. However, little is known about the strategies and processes of how they adapted to these extreme environments.
Candidatus Parvarchaeales, representing a DPANN archaeal group with limited metabolic potentials and reliance on hosts for their growth, were initially found in acid mine drainage (AMD). Due to the lack of representatives, however, their ecological roles and adaptation to extreme habitats such as AMD, as well as how they diverge across the lineage remain largely unexplored. By applying genome-resolved metagenomics, 28 Parvarchaeales-associated metagenome-assembled genomes (MAGs) representing two orders and five genera were recovered. Among them, we identified three new genera and proposed the names Candidatus Jingweiarchaeum, Candidatus Haiyanarchaeum, and Candidatus Rehaiarchaeum with the former two belonging to a new order Candidatus Jingweiarchaeales. Further analyses of metabolic potentials revealed substantial niche differentiation between Jingweiarchaeales and Parvarchaeales. Jingweiarchaeales may rely on fermentation, salvage pathways, partial glycolysis, and pentose phosphate pathway (PPP) for energy reservation, while the metabolic potentials of Parvarchaeales might be more versatile. Comparative genomic analyses suggested that Jingweiarchaeales are more favorable to habitats with higher temperatures and Parvarchaeales are better adapted to acidic environments. We further revealed that the thermal adaptation of these lineages especially for Haiyanarchaeum might rely on innate genomic features such as the usage of specific amino acids, genome streamlining, and hyperthermal featured genes such as rgy. Notably, the acidic adaptation of Parvarchaeales was possibly driven by horizontal gene transfer (HGT). Reconstruction of ancestral states demonstrated that both may originate from thermal and neutral environments and later spread to mesothermal and acidic environments. These evolutionary processes may also be accompanied by adaptation toward oxygen-rich environments via HGT.
This paper aims to present the exact closed-form solutions for the free vibration of double-beam systems composed of two parallel beams connected by an arbitrary number of discrete elastic supports. The general solutions of the mode shapes of the double-beam system are derived employing the Laplace transform method from a perspective of the entire domain of beams without enforcement of any segmentation. A unified strategy applied to various boundary conditions is proposed to determine the independent constants involved in the general solutions, as well as the frequency equation. Numerical calculations are performed to verify the present solutions by comparing the results from the previous literature and finite element simulation, and to discuss the effects of support parameters (stiffness, location, and number) on the modal characteristics of the double-beam system in detail. Outcomes show that the support location plays a pivotal role in regulating the modal characteristics of the double-beam system; for each-order mode, there are one or more potential optimal positions to maximize the effect of the elastic support. The mode veering phenomenon is detected as the support parameters change. It is highlighted that, by introducing an amplitude similarity index, the proximity degree for the mode shapes of the two beams influenced by the support parameters can be evaluated quantitatively. The present analysis is greatly helpful to the optimal design, health monitoring, and vibration control of the double-beam system.
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