Immobilized metal chelate affinity chromatography (IMAC) support was practiced for α -amylase immobilization. Poly(hydroxyethylmethacrylate-methacryloylamidotryptophan)-Ni 2+ [p(HEMA-MAT)-Ni 2+ ] nanospheres, average diameter 100 nm, were produced by surfactant free emulsion polymerization. Characterizations of p(HEMA-MAT)-Ni 2+ nanospheres were carried out by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). In addition, average particle size, size distribution, and surface charge were specified. The amount of N-methacryloylamidotryptophan (MAT) incorporated to polymer was determined as 1.95 mmol/g polymers by using nitrogen stoichiometry. The specific surface areas of poly(hydroxyethylmethacrylate) [p(HEMA)] and p(HEMA-MAT)nanospheres were calculated as 1856 m 2 /g and 1914 m 2 /g, respectively. Protein adsorption increased with increasing initial protein concentration and maximum α -amylase adsorption on p(HEMA-MAT)-Ni 2+ nanospheres was observed at pH 4.0. Both free and immobilized α -amylase showed pH optimum at pH 7.0. It was determined that the immobilized α -amylase had better thermostability than the free one. Immobilization of the enzyme did not significantly change the kinetic parameters. The storage stability of α -amylase increased upon immobilization. It was also observed that p(HEMA-MAT)-Ni 2+ nanospheres can be repeatedly used for α -amylase immobilization.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.