This study includes 10 samples isolated from a different source. This research paper was to study the antibacterial activity and antibiofilm activity of an alcoholic extract of Lemon Myrtle (Backhousia citriodora) leaves, against 10 clinical isolates of Pseudomonas aerogenoza. Isolates were diagnosed by the VITEK-2 compact system and (PCR). The capacity of isolates to produce biofilm was tested by using the Microtiter plate technique (96 wells). The results showed that isolates were able to form a biofilm, also the ability of Backhousia citriodora was been effective on bacteria and biofilm formation.
This study propose a rapid, suitable and sensitive method for using ATR-FTIR spectroscopy coupled with OMNIC software version 8.2.0.387 were used for detecting humidity effect of red grape juice quality. These results show that the physical parameters study, as well as humidity and time were present in the published red grape juice literature. compare with conventional analysis techniques as a high-throughput method for routinely screening red grape juice at factories, it offers many advantages. Concerning the small sample size, however, caution is required when drawing conclusions about the differences in storage ways between the red grape juices. Taking into consideration the continuous increase in the intake and output of red grape juice, this study will serve as a reference for future researches that could explore the effect of storage conditions and the general technology for the output of red grape juice.
Background: MIC is the lowest concentration of an antibiotic required to inhibit the growth of an organism. Methods: MIC was done against six conventional antibiotics e.g., Amoxicillin, Ceftriaxone, Gentamycin, Tetracycline, Ciprofloxacin, and Levofloxacin. E. coli O157:H7 was collected from hospital. The antibacterial activity of six conventional antibiotics was assessed against E. coli O157:H7by using the broth microdilution method then the fractional inhibitory concentration (FIC) index was used to define the interactions between antibiotics.
Results:The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of antibiotics against E. coli O157:H7 showed that the highest concentration appeared in ciprofloxacin was 62.5 µg/ml. while ceftriaxone and gentamycin were 15.62 µg/ml. amoxicillin and levofloxacin was 7.812 µg/ml. the lowest concentration appeared in Tetracycline was 0.976 µg/ml. The higher FICI was seen in Ceftriaxone + Levofloxacin combination 0.3 % followed by Levofloxacin + Ciprofloxacin (0.1). Conclusions: The antibacterial activity of both Ceftriaxone and Levofloxacin was enhanced by the combination which proved a highly synergistic effect against E. coli O157:H7.
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