Urine specimens were collected aseptically and inoculated directly onto CLED and MacConkey agar medium and incubated overnight. Single target colonies were sub cultured, and identification was based on culture, morphology, and biochemical characteristics. Enterococci isolates were subjected to biofilm assay (Tube method), and resistance pattern was determined for both biofilm-formers and non-biofilm-formers. Genomic DNA was extracted using the Bioneer kit, and the gene for virulence was detected by PCR and agarose gel electrophoresis. 148 Patients having one or more urinary symptoms were the study population. The aim of the study was to determine the multidrug resistant ability of biofilm-forming Enterococci. A number of growths were encountered; Enterococci accounted for only 9.46% (14), the highest prevalence was seen in the age group 21-30 (35.71%), and females (64.29%) were more prone to enterococcal infection than their males (35.71) counterpart. There is a relationship between biofilm production and antibiotic resistance because multidrug resistant Enterococci isolate produced bands against esp gene with an average of 510kbp. Biofilm forming strains showed the highest resistance to gentamicin and penicillin (83.3%), and vancomycin can be considered a good alternative therapy in enterococcal UTIs because of its lower resistance (75%).
Background: Wound infection comprises numerous different organisms that have the ability to surface colonization of wounds. Multidrug-resistant Pseudomonas aeruginosa is one of the pathogenic bacteria associated with wound infections. Aim: This study isolated and identified multidrug-resistant Pseudomonas aeruginosa from infected wounds and determine the antibacterial activity of Lawsonia inermis leaf extracts against it. Design: This is a Clinical and laboratory-based study involving patients with defined cases of wound infections. Place and Duration of Study: This study was conducted in the Microbiology (Bacteriology) laboratory of Specialist Hospital, Bauchi, Nigeria, from February to November 2021. Methods: Twenty-eight (28) Pseudomonas aeruginosa isolates were recovered from 179 wound swabs using standard laboratory procedures and were screened for multidrug-resistant patterns according to the Kirby-Bauer disc diffusion method. Antibacterial efficacy of the aqueous, ethanolic, and methanolic leaf extracts of Lawsonia inermis was tested against the multidrug-resistant isolates using agar well diffusion techniques. The zone of inhibition was measured and the differences between means were statistically analyzed (p<0.05). Results: A total of twenty-eight (28) multidrug-resistant Pseudomonas aeruginosa were confirmed, showing resistance to Amoxicillin (64.3%), Ceftazidime (85.71%), and Cefotaxime (78.57%) but sensitivity to Imipenem (95.5%). The phytochemical screening revealed the presence of flavonoids, glycosides, saponins, steroids, and tannins among others. MDR P. aeruginosa was inhibited at varied concentrations of the extracts with the diameter mean zone of inhibition increasing as the concentration increased. The Methanol extracts showed the highest antibacterial activity against MDR P. aeruginosa with a mean zone of inhibition of 9.500±0.288mm at 400mg/ml. Conclusion: These results indicated that Lawsonia inermis leaf extracts possess antibacterial activities on Multidrug-resistant Pseudomonas aeruginosa which could be a good source for the production of plant-based antibacterial drugs., although somewhat less than the synthetic standard drugs (Imipenem) having a mean of 13.83±0.288mm.
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