The current study was aimed at analyzing putative protein sequences of the protamine-like proteins of 12 Drosophila species based on the reference sequences of two protamine-like proteins (Mst35Ba and Mst35Bb) found in Drosophila melanogaster sperm nuclei. Protamine-like proteins belong to a larger group of proteins that are involved in DNAbinding known as sperm nuclear basic proteins (SNBPs). SNBPs play a role in chromatin condensation during the postmeiotic stage of spermatogenesis, termed spermiogenesis. During spermiogenesis, nuclear transformation occurs where histones are exchanged for SNBPs, the chromatin condenses, and the nucleus transforms into a needle-like shape in Drosophila. Our goal was to search the 12 sequenced Drosophila genomes for protamine-like proteins based on the known sequences for D. melanogaster. Searches were performed on genomic DNA, mRNA transcripts and amino acid sequences using NCBI basic local alignment search tool (BLAST). Sequence alignments and analysis of amino acid content indicate that homologs for Mst35Ba and Mst35Bb are present in all 12 species of flies analyzed in this study. Functional analyses of a conserved region found within the proteins indicate the presence of a DNA-binding domain, possibly a high mobility group DNA- binding box. This study represents the first large-scale, single-genus dataset for protamine-like proteins and provides the basis for a fine-grained analysis of their evolution.
The current study was aimed at analyzing putative protein sequences of the transition protein-like proteins in 12 Drosophila species based on the reference sequences of transition protein-like protein (Tpl (94D) ) expressed in Drosophila melanogaster sperm nuclei. Transition proteins aid in transforming chromatin from a histone-based nucleosome structure to a protamine-based structure during spermiogenesis - the post-meiotic stage of spermatogenesis. Sequences were obtained from NCBI Ref-Seq database using NCBI ORF-Finder (PSI-BLAST). Sequence alignments and analysis of the amino acid content indicate that orthologs for Tpl (94D) are present in the melanogaster species subgroup (D. simulans, D. sechellia, D. erecta, and D. yakuba), D. ananassae, and D. pseudoobscura, but absent in D. persmilis, D. willistoni, D. mojavensis, D. virilis, and D. grimshawi. Transcriptome next generation sequence (RNA-Seq) data for testes and ovaries was used to conduct differential gene expression analysis for Tpl (94D) in D. melanogaster, D. simulans, D. yakuba, D. ananassae, and D. pseudoobscura. The identified Tpl (94D) orthologs show high expression in the testes as compared to the ovaries. Additionally, 2 isoforms of Tpl (94D) were detected in D. melanogaster with isoform A being much more highly expressed than isoform B. Functional analyses of the conserved region revealed that the same high mobility group (HMG) box/DNA binding region is conserved for both Drosophila Tpl (94D) and Drosophila protamine-like proteins (MST35Ba and MST35Bb). Based on the rigorous bioinformatic approach and the conservation of the HMG box reported in this work, we suggest that the Drosophila Tpl (94D) orthologs should be classified as their own transition protein group.
Background: The menhaden, Bervoortia tyrannus, is one of the most important fish within the oceanic ecosystem and a crucial species supporting major fisheries along the Atlantic and Gulf coasts. However, little is known about menhaden from a genetic aspect. The objective of this project is to apply high throughput sequencing to the testes of menhaden to provide the genetic tools required to further study their population dynamics
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