Malignant tumor is a serious threat to human health. With the development of medical technology, a variety of treatment methods appear in clinic. As a non-invasive treatment, laser photothermal therapy is a treatment that kills cancer cells by converting light energy into heat energy through laser irradiation. Its advantage is protecting normal tissue while destroying cancerous tissue. However, it's still not clear that the effect of heat generated by laser on tissue and temperature changes during photothermal treatment process. Optical coherence tomography (OCT) is a non-contract, real-time optical imaging technology. OCT has been widely used in clinical treatment and scientific research based on fast imaging speed and high detection sensitivity. In our study, breast cancer of mice was chosen as the research object. Combined infrared thermography and OCT were applied to monitor the dynamic changes of tumor tissue. The effect of photothermal from OCT image and temperature were obtained and analyzed. Specifically, we investigated the structural change characteristics and temperature distribution of tumor tissue with increasing laser power. And then, the temperature change of tumors of different sizes at power of 3W were further analyzed. The results show that combined with OCT images and temperature can be well used to guide the photothermal treatment process. It can serve as a basis for the method with safely, consistently and effectively.
Organ development analysis plays an important role in assessing an individual’ s growth health. In this study, we present a non-invasive method for the quantitative characterization of zebrafish multiple organs during their growth, utilizing Mueller matrix optical coherence tomography (Mueller matrix OCT) in combination with deep learning. Firstly, Mueller matrix OCT was employed to acquire 3D images of zebrafish during development. Subsequently, a deep learning based U-Net network was applied to segment various anatomical structures, including the body, eyes, spine, yolk sac, and swim bladder of the zebrafish. Following segmentation, the volume of each organ was calculated. Finally, the development and proportional trends of zebrafish embryos and organs from day 1 to day 19 were quantitatively analyzed. The obtained quantitative results revealed that the volume development of the fish body and individual organs exhibited a steady growth trend. Additionally, smaller organs, such as the spine and swim bladder, were successfully quantified during the growth process. Our findings demonstrate that the combination of Mueller matrix OCT and deep learning effectively quantify the development of various organs throughout zebrafish embryonic development. This approach offers a more intuitive and efficient monitoring method for clinical medicine and developmental biology studies.
Due to the advantages of high sensitivity, high resolution and nondestructive in vivo three-dimensional detection, Optical Coherence Tomography has been widely studied in various fields such as biology, genetics and medicine. Zebrafish is a kind of freshwater fish, whose embryos are easy to reproduce in large numbers and have high transparency for observation.In particular, the genetic homology between zebrafish and human is as high as 70%, which makes zebrafish gradually become an excellent model for studying human development or various serious diseases. In this study, a method for continuous observation of zebrafish embryos using OCT was proposed. In this experiment, the development of zebrafish embryos before hatching (0dpf-3dpf) was continuous observed by OCT, and the proportion of yolk sac to embryo volume was extracted and quantified. The proportion of embryos collected by OCT was compared with the proportion of zebrafish embryos observed by microscope. All experiments were repeated three times. The results show that the method of quantification of zebrafish embryo development by OCT can not only observe the internal development structure of the embryo, but also calculate the volume proportion of embryo development more accurately than microscope. This method provides a more rapid and precise important means for early clinical judgment of embryo development.
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