Drought is one of the most critical environmental factors constraining maize production. When it occurs at the flowering stage, serious yield losses are caused, and often, the damage is irretrievable. In this study, anthesis to silk interval (ASI), plant height (PH), and ear biomass at the silking date (EBM) of 279 inbred lines were studied under both water-stress (WS) and well-water (WW) field conditions, for three consecutive years. Averagely, ASI was extended by 25.96%, EBM was decreased by 17.54%, and the PH was reduced by 12.47% under drought stress. Genome-wide association studies were carried out using phenotypic values under WS, WW, and drought-tolerance index (WS-WW or WS/WW) and applying a mixed linear model that controls both population structure and relative kinship. In total, 71, 159, and 21 SNPs, located in 32, 59, and 12 genes, were significantly (P < 10−5) associated with ASI, EBM, and PH, respectively. Only a few overlapped candidate genes were found to be associated with the same drought-related traits under different environments, for example, ARABIDILLO 1, glycoprotein, Tic22-like, and zinc-finger family protein for ASI; 26S proteasome non-ATPase and pyridoxal phosphate transferase for EBM; 11-ß-hydroxysteroid dehydrogenase, uncharacterised, Leu-rich repeat protein kinase, and SF16 protein for PH. Furthermore, most candidate genes were revealed to be drought-responsive in an association panel. Meanwhile, the favourable alleles/key variations were identified with a haplotype analysis. These candidate genes and their key variations provide insight into the genetic basis of drought tolerance, especially for the female inflorescence, and will facilitate drought-tolerant maize breeding.
Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length (7.23 cm), root length (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt. (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.
Pakistan is one of the most severely affected countries by Global climate change, it is an agriculture based country and its economy (21%) mainly depend on agriculture production. Wheat is the major staple food crop in Pakistan and takes key position in the national economy. It contribute 12.5% share in agriculture and 2.9% in the country’s GDP. Frequent droughts and scarcity of the water severely affecting the wheat production. To fulfill the feed requirements of rapidly growing population, it is necessary to explore the advanced genetic resource that can be able to perform better in changing climate. Six wheat genotypes were tested for their early seedling and physiological performance under different water stress environments. The seeds of six wheat genotypes (Khirman, Chakwal-86, MSH-36, DH-3/48, NIA Amber and NIA-10 10/8) were tested for physiological characterization under pot house experiment for individual genotypic response to water stress. The variance of analysis shows two-way interaction water stress [Control (normal four irrigations) and terminal drought (Soaking dose) and wheat genotypes (P≤ 0.05). Seven physiological indices, including Proline content, Glycine-betaine, Total sugars, Total chlorophyll, Nitrate Reductase Activity ((NRA), Potassium (K+) content, and Osmotic potential (OP) were used to evaluate the drought tolerance of six wheat genotypes. From the current data it was illustrated that, MSH-36 and DH-3/48 exhibited the tolerance followed by, Khirman and Chakwal-86 by maintaining their osmotic potential and accumulation of higher proline and glycine-betaine content that helpful for plant to enhancing their tolerance under water stress and to maintain their growth and development, whereas NIA Amber and NIA-10 10/8 are the drought sensitive genotypes as they could not maintain their osmotic potential under drought stress environment.
Drought is one of the most critical environmental factors constraining corn production especially when it occurs during flowering, resulting in serious yield losses. In this study, anthesis to silk interval (ASI), plant height (PH), and ear biomass at the silking date (EBM) of 279 inbred lines were evaluated under water-stress (WS) and well-water (WW) field conditions for three consecutive years. Averagely, ASI was extended by 25.96%, ear biomass was decreased by 17.54%, and the PH was reduced by 12.47% under drought stress conditions. Genome wide association studies (GWAS) were carried out using phenotypic values under WS, WW and drought-tolerance index (WS-WW or WS/WW) applying mixed linear model controlling both population structure and relative kinship. Totally, 71, 159, and 21 SNPs were significantly (P < 10-5) associated with ASI, ear biomass, and PH, respectively. Candidate genes encoding ARABIDILLO 1 protein, glycoprotein, Tic22-like and Zinc finger family protein for ASI, and 26S proteasome non-ATPase regulatory subunit-9 for EBM, were identified under both WW and WS conditions. Pyridoxal phosphate transferase was associated with EBM under drought stress treatment in consecutive two years. Furthermore, most candidate genes were evidenced to be drought responsive in the association panel. Meanwhile, the favourable/drought tolerance haplotypes were identified based on haplotype analysis. These findings provide insights into the genetic basis of drought tolerance at the flowering stage especially for the female inflorescence development and will facilitate high drought tolerant maize breeding.
Grafting is an ancient agricultural technique that is frequently used to enhance the performance of horticultural plants, including vegetables and woody fruit trees. For successful grafting, genotypes of the compatible scion (the upper part) and the rootstock (the lower part) must interact. Molecular signals, including nutritional and hormonal signals, proteins, and messenger RNAs (mRNAs), are known to be transferred from the rootstock to the scion and vice versa. Nonetheless, there are still numerous mysteries regarding artificial grafts, including the occurrence of genetic/epigenetic alterations due to exchanges between the graft partners, and the long-term ramifications of these alterations on the phenotype are unknown. Recent studies on the interactions between rootstocks and scions suggest that grafting responses have an epigenetic component. In this review, we focus on the current knowledge of epigenetic consequences following grafting. Epigenetic regulations are known to regulate chromatin architecture, alter gene expression, and affect cellular function in plants. Mobile small RNAs, for example, have been shown to modify the DNA methylation pattern of the recipient partner across the graft union. More recently, mRNA 5-methylcytosine (m5C) modification has been shown to elucidate the long-distance transport mechanism of grafting in Arabidopsis thaliana. We also discuss how grafts can cause heritable epigenetic alterations that result in novel plant phenotypes, and how this might help increase horticultural crop quality, yield, and stress resistance in the context of climate change.
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