Ticks transmit pathogens to animals and humans more often than any other arthropod vector. The rural economy of Pakistan mainly depends on livestock farming, and tick infestations cause severe problems in this sector. The present study aimed to molecularly characterize the Anaplasma spp. in hard ticks collected from six districts of Khyber Pakhtunkhwa, Pakistan. Ticks were collected from various livestock hosts, including cattle breeds (Holstein-Friesian, Jersey, Sahiwal, and Achai), Asian water buffaloes, sheep, and goats from March 2018 to February 2019. Collected ticks were morphologically identified and subjected to molecular screening of Anaplasma spp. by amplifying 16S rDNA sequences. Six hundred seventy-six ticks were collected from infested hosts (224/350, 64%). Among the nine morphologically identified tick species, the highest occurrence was noted for Rhipicephalus microplus (254, 37.6%), followed by Hyalomma anatolicum (136, 20.1%), Rhipicephalus haemaphysaloides (119, 17.6%), Rhipicephalus turanicus (116, 17.1%), Haemaphysalis montgomeryi (14, 2.1%), Hyalomma dromedarii (11, 1.6%), Haemaphysalis bispinosa (10, 1.5%), Hyalomma scupense (8, 1.2%), and Haemaphysalis kashmirensis (8, 1.2%). The occurrence of tick females was highest (260, 38.5%), followed by nymphs (246, 36.4%) and males (170, 25.1%). Overall, the highest occurrence of ticks was recorded in the Peshawar district (239, 35.3%), followed by Mardan (183, 27.1%), Charsadda (110, 16.3%), Swat (52, 7.7%), Shangla (48, 7.1%), and Chitral (44, 6.5%). Among these ticks, Anaplasma marginale was detected in R. microplus, R. turanicus, and R. haemaphysaloides. The 16S rDNA sequences showed high identity (98–100%) with A. marginale reported from Australia, China, Japan, Pakistan, Thailand, Uganda, and the USA. In phylogenetic analysis, the sequence of A. marginale clustered with the same species reported from Australia, China, Pakistan, Thailand, Uruguay, and the USA. Further molecular work regarding the diversity of tick species and associated pathogens is essential across the country.

Hard ticks (Ixodida: Ixodidae) are medically important ectoparasites that feed on all classes of terrestrial vertebrates. Recently, we molecularly characterized hard ticks and associated Anaplasma spp. in the northern and central regions of Khyber Pakhtunkhwa (KP), Pakistan; however, this knowledge was missing in the southern regions. This study aimed to investigate tick prevalence, host range, genetic diversity, and molecular survey of Anaplasma spp. in a wide range of tick species in two distinct physiographic regions of southern KP. A total of 1873 hard ticks were randomly collected from 443/837 hosts (cattle, Asian water buffaloes, horses, goats, sheep, dogs, and camels) in Lakki Marwat, Bannu, and Orakzai districts of KP. Overall, 12 tick species were morphologically identified, among which Hyalomma dromedarii was the most prevalent species (390/1873, 20.9%), followed by Hy. anatolicum (294, 15.7%), Rhipicephalus microplus (262, 14%), Hy. scupense (207, 11.1%), R. sanguineus (136, 7.3%), R. turanicus (121, 6.5%), Haemaphysalis cornupunctata (107, 5.7%), R. haemaphysaloides (110, 5.9%), Ha. montgomeryi (87, 4.6%), Hy. isaaci (58, 3.1%), Ha. bispinosa (54, 2.9%), and Ha. sulcata (47, 2.5%). The extracted DNA from a subset of each tick species was subjected to PCR to amplify cox1 or 16S rRNA sequences of ticks and 16S rRNA sequences of Anaplasma spp. The tick cox1 sequences showed 99–100% identities with the sequences of the same species, whereas 16S rRNA sequences of R. turanicus, Ha. montgomeryi and Ha. sulcata showed 97–100% identities with the corresponding species. The 16S rRNA sequence of Ha. cornupunctata showed 92% identity with the species from the same subgenus, such as Ha. punctata. The 16S rRNA sequence of Anaplasma spp. showed 100% identity with Anaplasma marginale. Moreover, 54 ticks were found positive for A. marginale with a total infection rate of 17.2%. The highest infection rate was recorded in Hy. dromedarii (31.1%) and the lowest in each R. haemaphysaloides and R. sanguineus (20%). All the cox1 or 16S rRNA sequences in phylogenetic trees clustered with the same species, except Ha. cornupunctata, which clustered with the Ha. (Aboimisalis) punctata. In this study, Ha. cornupunctata was reported for the first time at the molecular level. The genetic characterization of ixodid ticks and molecular detection of associated A. marginale will assist in the epidemiological surveillance of these parasites in the region.

Escalating pesticide deposition in the aquatic environment is becoming a potential threat to aquatic organisms. Present study was designed to investigate acute toxicity in O. niloticus. the fish were exposed to five different concentrations of malathion, chlorpyrifos and lambda-cyhalothrin (synthetic pyrethroid) pesticides in the laboratory using separate aquaria for each chemical. Mortality ranged from 100 to 0%. The 24 h LC50 (median lethal concentration) for O. niloticus was 5.70, 0.5 and 0.015 ppm for malathion, chlorpyrifos and Lambda-cyhalothrin, respectively. LC50 values obtained after 24 h of exposure indicated that the fish were sensitive to all the three tested pesticides. The mortality rate was directly proportional to the pesticides concentration. The results showed that the lambda- cyhalothrin was found more toxic followed by chlorpyrifos and malathion. The study results suggested that the runoff of organophosphates and synthetic pyrethorid pesticides from nearby agricultural fields to aquatic environment posed health hazards on fish.

Purpose Polymer substrates with micron size roughness features have been found to play an important role in the mechanical performance of thin functional films which are used extensively in stretchable and flexible micro electromechanical systems. The purpose of this study is to report the stretchability and flexibility limits of micro size silver nano platelet films on a soft polymer substrate having two different orientations of micro grating with respect to the applied load. Design/methodology/approach Parallel and perpendicular micro gratings on the surface of a soft polymer substrate polydimethylsiloxane were patterned using a carefully machined master aluminum block and thin aluminum foils. Silver nano platelet-based films were rod coated on the substrate surface containing the micro gratings. These films were dried in ambient air and were tested for their stretchability and flexibility limits using homemade tools. Finite element modeling has also been performed and was found to support the experimental observations. Findings Experiments indicate that stretchability of silver nano platelet-based thin films tends to increase when the grating orientation remains parallel to the axis of the applied load, while its flexibility improves when the orientation becomes perpendicular to the loading axis. Originality/value The effect of grating orientation with respect to the applied load was investigated. The experiments show that micro grating roughness features are capable of enhancing the mechanical performance of nano platelet-based silver films on a soft polymer substrate and can be used in various stretchable and flexible micro electro mechanical device applications.

Background: One of the most potentially fatal pediatric events is gastrointestinal (GI) bleeding. Lower gastrointestinal bleeding children often have anemia, but determining the cause of the bleeding is crucial in developing a management plan. Objective: To assess the causes and outcome of children presenting with lower gastrointestinal bleeding. Methodology: The current study was prospective cross sectional study carried out at the pediatrics department of Fauji Foundation Hospital, Peshawar Cantt for duration of one year from June 2021 to July 2022. A total of 250 children with lower GI bleeding were enrolled in the current study. All the data was analyzed by IBM SPSS version 23. Results: In the current study, male children were 140 (56%) while female children were 110 (44%) with the mean age of 6.80 (± 1.50) years. The commonest complaint was abdominal pain in 105 (42%) patients whereas the commonest clinical observation was pallor observed amongst 115 (46%) patients. Juvenile colonic or rectal polyps were observed in 91 (36.4%) patients based on colonoscopy. Based on histo-pathological findings, polyps were observed in 93 (37.2%) patients, amongst whom 90 (96.77%) cases were Juvenile polyps and 3 (3.33%) cases were familial adenomatous polyposis. Conclusion: There are several underlying causes of lower gastrointestinal bleeding (LGIB). Abdominal pain was a common symptom in children with LGIB while the most typical cause of LGIB in children was a juvenile polyp in our study. Colonoscopy is a safe and effective diagnostic and therapeutic technique for children with LGIB. Keywords: Children; Lower gastrointestinal bleeding; Causes; Outcomes