Background Theoretical frameworks have shown that stress might influence working memory in different ways. Previous research has investigated the effect of stress on female's working memory but there is lack of evidence regarding the impact of emotional aspects. Objectives This study examined the effect of stress induction on auditory working memory (AWM) performance among university students for emotional (positive and negative) and non-emotional (neutral) stimuli. Methods A sample of 102 female students at the Universities of Isfahan, Iran was selected using convenience sampling in 2018. Participants completed the demographic information sheets, then, they were randomly assigned into the experimental and control groups. The stress was induced by the Socially Evaluated Cold Pressor Test (SECPT). An n-back task was presented pre and post of stress induction, to evaluate the AWM performance (accuracy and reaction time). The research data were analyzed using mixed-model ANOVA. Results Both accuracy and reaction time (RT) scores were found to be enhanced for positive words in the experimental condition. However, accuracy and RT indices were found to be worsening for negative words in the experimental condition. Conclusions This study supports the idea that stress influences AWM performance depend on emotionally-valenced stimuli, which may help us to better understand the underlying mechanisms of memory processing.